FastQCFastQC Report
Mon 20 Feb 2023
HTGHVDRX2_n02_EN74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGHVDRX2_n02_EN74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34613889
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12698813.6687036235656736No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1016000.2935237932958068No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA988480.28557322755614084No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG759800.21950726195487597No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC593040.1713300692678595No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT552070.15949378008348036No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG548530.1584710692288867No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG496340.1433933066579141No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAAC481960.13923890493784158No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC460530.13304774854972234No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA420990.12162458832637962No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC417200.12052965212894742No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA412760.11924693004013505No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA373480.10789888417334441No Hit
AAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTC346520.10011010320163677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)