FastQCFastQC Report
Mon 20 Feb 2023
HTGHVDRX2_n01_EN98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGHVDRX2_n01_EN98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12508402
Sequences flagged as poor quality0
Sequence length151
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1056990284.50241685548642TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTAT288890.2309567601041284TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA241800.1933100647069066TruSeq Adapter, Index 7 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG221520.1770969625056822TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACCGGCTATGATCTCGTATG217840.17415494001551918Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA130390.10424193274248782TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)