FastQCFastQC Report
Mon 20 Feb 2023
HTGHVDRX2_n01_EN97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGHVDRX2_n01_EN97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26732153
Sequences flagged as poor quality0
Sequence length151
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2225221683.24139099458245TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTAT664580.24860698650048876TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA479540.179386972684168TruSeq Adapter, Index 7 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACCGGCTATGATCTCGTATG429450.16064923764277422Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG428440.16027141547484036TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)