FastQCFastQC Report
Mon 20 Feb 2023
HTGHVDRX2_n01_EN95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGHVDRX2_n01_EN95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30250754
Sequences flagged as poor quality0
Sequence length151
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT2741530690.62685181334653TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA687190.22716458571578083TruSeq Adapter, Index 7 (96% over 32bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG624670.20649733226484204TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACCGGCTATGATCTCGTATG567040.18744656744754196Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTAT563810.18637882546663134TruSeq Adapter, Index 7 (97% over 36bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA398580.1317586993038256TruSeq Adapter, Index 7 (97% over 34bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA333080.11010634644015814TruSeq Adapter, Index 2 (96% over 33bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT332620.1099542841147034TruSeq Adapter, Index 2 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATAATCTCGTAT323920.10707832274197199TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)