FastQCFastQC Report
Mon 20 Feb 2023
HTGHVDRX2_n01_EN94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGHVDRX2_n01_EN94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16071888
Sequences flagged as poor quality0
Sequence length151
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT1367279985.0727618310929TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCGCGTAT439200.2732721880590507TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA308190.19175718496793906TruSeq Adapter, Index 7 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACCGGCTATGATCTCGTATG267900.16668856826279527Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG244800.15231564580340531TruSeq Adapter, Index 7 (97% over 35bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTA160930.10013135980041672TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)