FastQCFastQC Report
Mon 20 Feb 2023
HTGHVDRX2_n01_EN62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGHVDRX2_n01_EN62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20967466
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT997423547.570054483455465TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTAT328600.15671898549877225TruSeq Adapter, Index 13 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG288270.13748442467964417TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACATTCAGAAATCTCGTATG220640.10522969251506117Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)