FastQCFastQC Report
Mon 20 Feb 2023
HTGHVDRX2_n01_EN60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGHVDRX2_n01_EN60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31581369
Sequences flagged as poor quality0
Sequence length151
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2884221391.3266711142256TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCGCGTAT902790.285861578704837TruSeq Adapter, Index 13 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACATTCAGAAATCTCGTATG675510.21389509745445168Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTA624310.19768300734524838TruSeq Adapter, Index 13 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAAAAATCTCGTAT575850.1823385173707954TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT539920.17096155647970804TruSeq Adapter, Index 27 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG496300.15714961564839067TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAACACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT392090.12415231271323292TruSeq Adapter, Index 27 (97% over 37bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTA341850.10824419929357718TruSeq Adapter, Index 13 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAATCACATTCAGAAATCTCGTAT333800.10569522809476689TruSeq Adapter, Index 27 (97% over 37bp)
GATCGAAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT315850.10001149728499736TruSeq Adapter, Index 27 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)