FastQCFastQC Report
Mon 20 Feb 2023
HTGHVDRX2_n01_EN36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGHVDRX2_n01_EN36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57369123
Sequences flagged as poor quality0
Sequence length151
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2154999037.56374313060355TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGAT19675633.42965500797354TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTTT6549741.1416838287731887TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTGT1676840.2922896346175625TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGTT924890.1612173851777375TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGCGTAT686910.11973514045177229TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCTCCGGAGAATCTCGTAT671650.11707517299854836TruSeq Adapter, Index 3 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)