FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42806842
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3792930.8860569532319156No Hit
GGTTTAAATTTTCAACTAATGCATTGTACAGCTCCATGTTTATTTGCCGA614860.14363591689384608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA78150.062.40995856
TCGTCTC78600.062.4098455
CCGATTG79350.062.31145547
GCCGATT81100.060.793946
CTCCATG81450.060.59181232
TCCGGGA28450.060.44057518
GCTCCAT82000.060.0168931
CCGGGAC28450.059.9504119
GGACGAT38300.059.8175628
CGGGACT28200.059.49483520
TGTACAG85900.057.8323325
GTCCGTA35750.057.7162938
CTTGGAC39300.057.6765625
GTCTCAA85550.057.17576257
TCCGTAG36150.056.90869
TTGGACG40350.056.52187326
CGTCCGT36550.056.4380047
AGCTCCA90100.055.99120330
GGGACTT30300.055.9512521
ATACGTC37650.055.8513764
Produced by FastQC (version 0.11.5)