FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n02_1751_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n02_1751_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11514138
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTAAATTTTCAACTAATGCATTGTACAGCTCCATGTTTATTTGCCGA2612432.2688889085748323No Hit
GCCATACGTCCGTAGTATGGACCCTGCAGGAATGGTCTACATTTTCTATC1362191.183058601521017No Hit
GCTTTGTACATCTGTCATGGCATTCTTGGACGATTCTGTATACTTCGGAA1239041.0761031351196242No Hit
CCCATATGCTGATCTTTTCCGGGACTTCTAATGACTAATGTTTTGTTGTA1000720.8691228123199496No Hit
GTACCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTA851840.7398209053947417No Hit
CGTTTGTTTTTAATTATATACAGGTTGGTTAGGTGACGTTGGAGAAAAGA793800.6894133108357742No Hit
AGAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAA782430.6795384943275824No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTTGG761270.6611610873519146No Hit
CCATAACCGTTACCCTCCAATTACCCATATCCAACTCCACTACCACTTAC759070.6592503928648414No Hit
GGAGCGTTAACACACCATCCATGTGGCCCTTGAATTCCTGAATCCTGCAG537530.4668434580165706No Hit
CCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGC521590.45299960796023114No Hit
ATATTACAACGTCTCCAATGATATCAGCGGTTTTCAGAACAGAATGGGGC516360.4484573660659617No Hit
GACTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAAT467760.4062483878515265No Hit
ACATTTATTTTTTGAAAGAAAGGGGAAGATAGTTATATTAACACCAGAAT457390.39724206883745883No Hit
GTTGAAAAAATCGAGGTGGAATTTGAGAACGACAATTACTTAGCACTACA436900.3794465551828543No Hit
GGAACTGTCTGTTGGCAACGATAGAGCCAATTCTACCCTTCCTATCGGTT422110.3666014772447577No Hit
GCTTTTGTATTAGAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATA410760.3567440306864482No Hit
GATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAAC410350.3563879467138573No Hit
CCCTGCAGGAATGGTCTACATTTTCTATCTACTAGTAAAGCTTTATTCAC401140.34838908479297365No Hit
GCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAT382630.33231319617673505No Hit
AACCCGTACTTCCAACTGCAGGACGACCCAACCATCAAAGACACACCAGA376580.3270587863372838No Hit
GGATTTATGGATCAAACAACTGTCTCGGACAAGGCAATTATTTTCAAATA332160.28848012764828773No Hit
GATATTGGAATTGGAAATGGCAATTCACTTCCATTTATTTCAGAAATAGT318900.27696385087620107No Hit
GAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAAG304790.2647093512341089No Hit
GTTTTATTTCCGTGCATATGCATCCGACTTGCAATTAGTCGAAAGATTTC304610.26455302168516653No Hit
GTTCCTATGATGCCCCAGGCTCAAACTTCAGCAAGTATTCAGCCTCACGC302020.2623036131753849No Hit
GTGATATATCTACGGTAGGAGACGGTACTGAAGACTGAGGAGGTGATGGT297200.2581174552537064No Hit
GTTCTGGTATGACGCGTACTTGCTATATGATGCAACTGTCGTTCTCAACG294610.2558680467439247No Hit
CCTTTTTCTTTGAGATAGGATGCTATTGCCATGGAAACTGGGTGTTTTAT286280.24863346261787034No Hit
CTTTAAACATGGACCTACGCTTAGCCTGCGCTTAGCCTACAACTTCTTCC279320.242588720058766No Hit
GGATAATCAGCTCTGTCGTGACTGATAAATGCTATATCCGGCATATGCAG279240.2425192402592361No Hit
GGATCAGATAAGTCTATCGGTTTGCAAATTTTACCTATTAGCGGTATCGG269760.23428588401493886No Hit
GCATTATCAAGCATATCTACTGGAAACCAGAGGAAATTTGAGGTAAAACA266030.23104638836185568No Hit
CACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCT258260.22429816283251078No Hit
CTAACAAATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATT254660.22117157185366373No Hit
CACACCACACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAA253780.22040729405883447No Hit
GTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAATAATAC252270.21909586284270693No Hit
CCTCAGTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAA244050.21195681344100617No Hit
GGTTTTGAAATCCATCTGGGATGGCCGCCAGTGAGATTATCTTATTAGGT241800.21000269407922678No Hit
GCATATCAATGTACCAAAACAATAAAATGGTTTGAACCAAACATACTGTA229230.19908568057808584No Hit
CTCTTTTGTATAGGGCGATGAATTCTGCCTGGTTGACAGTGCCAAACCTG228390.19835614268302154No Hit
AGTCCTTCCGGGCTATTTGACCGGACCAACTCTATAATCAGCCTTGATCC224850.19528166155382193No Hit
CTTTTATGACCGTACTTTTAGAGCATCCACTGGGGCCAGACAGTAGTAGG223660.19424814953581415No Hit
CCTTTATTTAGCATGGCCATTGAATGTAACAATTATATATATCGCAAGCA221220.19212901565015114No Hit
GTTAAACTCCTTTACAATATCTGGACAGACGTAACAGTATTCTTGCTTAA210300.1826450230143151No Hit
CCATCCCGGTTGATCCTGAAAAGGTTGTTGCGATTGTGGAGTCCACCATG208030.1806735337026532No Hit
GCATGGCCATTGAATGTAACAATTATATATATCGCAAGCACAAAAAATCA206530.17937078746146695No Hit
CCTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTATT197650.17165852971364423No Hit
GAATGGGGCTGTTCTTCTTTATACTAACGTACTTCGGTTTTGTTACATTC197270.17132850066587701No Hit
CACACCACACACCACACACTACCCCTAACACTACCCTATTCTAACCCTGA189030.16417208131429378No Hit
GAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATAGGATGCTATTGC187850.16314725427122725No Hit
CTTAACTATCTGCTTAGTCGAGGAGAACCAGGATTCTGTTCGTTGCTCAG174270.1513530583010209No Hit
CCCTGTAGTGTGGTAGCCTATTTAGTTCATTCTCAGTCAACTGTAATATC174000.15111856397760737No Hit
GGGTGTTTTATTCCTTCAGTTAATCCAAGCAACAAAGATTGAGAATTATG173350.15055404060642663No Hit
CGTCTAAAGACATGGGGTCAATGCCTTTTTGGTTAATGATAACAAAACCC170130.14775747867534678No Hit
ATTTCCATGGTGCACAGTATCTTAACTATCTGCTTAGTCGAGGAGAACCA169350.1470800506299299No Hit
CCTTCTACCCGCTTACCAGTCACAGCTTTTGTATTAGAAACATTTTGAGC168130.1460204836870984No Hit
GAACCATTAACACTAAAACAGAATACAGAATATCCTTGTTCAAGGGCTTT165160.1434410461295496No Hit
CAAGAGTATCCTACTGCACATGATAACTTTAACTTTGGGATCTAATAAGT164300.1426941382846028No Hit
CTCAACCATCCACTCCCAACCACCATCCATCTCTCTACTTACCACTAACC163200.1417387910410662No Hit
GAGGACCATCGAATTGGAACTGTCTGTTGGCAACGATAGAGCCAATTCTA161910.140618429273646No Hit
GCTTCGAAAGGACGTTCCGTTGCTGTTTTGTCTGTATTTAGGAACTAAGA161250.14004522092752406No Hit
GACCAACTCTATAATCAGCCTTGATCCGCTGAGTGAGACGCTAACATCAA160770.13962834213034445No Hit
CAGTAATCACGCACTCCAAAATCTCCAAACAAATAAAATCGAAGCCTCAA160640.1395154374561083No Hit
CACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGAT159720.13871641976151405No Hit
CCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTACCC157840.1370836444725606No Hit
GTCCGACTAAACCGGACAAGAGTATCCTACTGCACATGATAACTTTAACT152510.13245455282887872No Hit
ATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATTTTTTCAC151960.13197687920711043No Hit
CCAATAAACTTGCAGACCAAGAGGTTGTTGACCTCATCACCTCTACGGTG151560.13162948020946075No Hit
GAGTTGAGCTGATAGTTCTTCCCCCTCTAGCCATCGATGTCAACAATAAC150810.13097810708886762No Hit
GCTTTTGAATCGAGGGGTGGATCCAAAACCTGGGGTAGCCAGACTTTAAA144890.12583660192365245No Hit
CCGTAGGAAGAACATTTCTTGATCACAGACTCAATTGCATTGTAACCTCT144570.12555868272553272No Hit
GGTTTTTGAATAATTTCGCCAACACTAGATCGTTTGGTGATGTCGCTGGG143800.12488993965505711No Hit
GAACTACCACTTTGTTCATGTGTACAATGTTCATTATCTCCATAAGCAAA143360.12450780075764246No Hit
CCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCTGTCT143150.1243254162838764No Hit
CCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGTT142780.12400407221105043No Hit
CCCCTTATTTCAACGTTATGGATCCCAGATAAAATACATTACTCCAAGAA141140.12257973632068678No Hit
AAATCAAGGAGAGAGAACTACCACTTTGTTCATGTGTACAATGTTCATTA132320.11491958842251151No Hit
GAAATATTATAAAAAACGTTCAAAAATATTTCGAATTTGGGTAGCTTCCA130990.11376448675532636No Hit
GTATGCTCTAATAACAGAAAATACAATAACAAGGAGGGAAGACCAATTTG127460.110698690601068No Hit
GTTCCCCGTGAGTTGCTTTCTCTGAAAACCTTATTATACATTAGAGTTAT126240.10963912365823653No Hit
GGGTGTCGTAATATGTAATAGACATGTGATACATTTTTATGTACACTCTA125700.10917013501140944No Hit
CGTACTTGCTATATGATGCAACTGTCGTTCTCAACGTCAACCCGTACTTC124970.1085361318406988No Hit
CCCTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTAT124500.10812793801846043No Hit
GGATACTTCTCAATCAACTTTACAAAGTGATATGAACATTCCCCGCATGG123690.10742445504821985No Hit
CTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAATAT121640.10564403518526527No Hit
GAGTTATGAAATTCGCTTACAGAAATGGTGAGCGATCCGTTGATATCATT120720.10484501749067103No Hit
GAACCTATAATTGCTTTTTGTTGAGGACTTATAGTGCTAGAAATGGCTTC120590.10473211281643488No Hit
GCGACTGTCTGTCCCCTTCAAGCACAACATAGAAAACAGAAGGATGTCTC119820.10406336974595927No Hit
GGTCCCAATCGAAATTTGGAGGCTGAAACAATGCTATGTCTCTATTGCGC117300.10187475606076633No Hit
CCAATTGTTCCCCGTGAGTTGCTTTCTCTGAAAACCTTATTATACATTAG117230.10181396123617764No Hit
GAATCTGAGGATTTTGGCAATGCAGACATGTACTTCTGGCACAAAATTTG116700.10135365756429184No Hit
GCTTTCCATCTGAGGAAGATCGTTGACTATACAATCACCTCTAAACTCGG116320.10102362851652463No Hit
GATTCTGCATTGAGCAAAGAATCATAGTTCCAGTCTAAGAAATCTTCCAT116030.10077176424322865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTC289450.065.3226955
GTACAGC291850.065.2009126
GCCGATT289800.065.1974746
CGTCTCA291250.064.9530656
CCGATTG291250.064.9329747
GCTCCAT293850.064.4503731
CTCCATG294100.064.44179532
TGCCGAT293750.064.3310145
TCCGGGA115350.063.81345418
CGGGACT115700.063.62317720
GGACGAT140650.063.58835628
CCGGGAC116000.063.45588319
GTCATGG141950.063.09586714
TTGCCGA302000.062.82287244
TTATATG303100.062.48769866
GTCTCAA304350.062.2838157
TTCGTCT304300.062.19241754
GGGACTT119800.061.59237721
TGTACAG310050.061.4639325
GGAGCGT67550.061.1922261
Produced by FastQC (version 0.11.5)