FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n02_1747_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n02_1747_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8124969
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTAAATTTTCAACTAATGCATTGTACAGCTCCATGTTTATTTGCCGA1751942.1562420730466787No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTTGG1437231.7689052105921883No Hit
CCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGC905001.1138504036138477No Hit
CCATAACCGTTACCCTCCAATTACCCATATCCAACTCCACTACCACTTAC748520.9212588995724169No Hit
AGAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAA691180.8506863226185848No Hit
GCTTTGTACATCTGTCATGGCATTCTTGGACGATTCTGTATACTTCGGAA680820.8379355047385412No Hit
GCCATACGTCCGTAGTATGGACCCTGCAGGAATGGTCTACATTTTCTATC642150.7903414769951737No Hit
CTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGA641380.789393781071657No Hit
GCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAT576710.7097996312355161No Hit
CCCATATGCTGATCTTTTCCGGGACTTCTAATGACTAATGTTTTGTTGTA566190.6968518895272092No Hit
GTACCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTA520440.6405439823831943No Hit
ATATTACAACGTCTCCAATGATATCAGCGGTTTTCAGAACAGAATGGGGC482070.5933191868178205No Hit
GTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAATAATAC457000.5624636844768269No Hit
GAACTACCACTTTGTTCATGTGTACAATGTTCATTATCTCCATAAGCAAA416500.5126173404476989No Hit
GATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAAC373130.45923867524909945No Hit
ACATTTATTTTTTGAAAGAAAGGGGAAGATAGTTATATTAACACCAGAAT336880.41462312040821325No Hit
GAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAAG306560.3773060549523327No Hit
CTTTTATGACCGTACTTTTAGAGCATCCACTGGGGCCAGACAGTAGTAGG305190.3756198946728289No Hit
AAATCAAGGAGAGAGAACTACCACTTTGTTCATGTGTACAATGTTCATTA292840.3604198366787615No Hit
GGAACTGTCTGTTGGCAACGATAGAGCCAATTCTACCCTTCCTATCGGTT284510.3501674898698075No Hit
CGTTTGTTTTTAATTATATACAGGTTGGTTAGGTGACGTTGGAGAAAAGA283400.34880133081123144No Hit
GAATGGGGCTGTTCTTCTTTATACTAACGTACTTCGGTTTTGTTACATTC267740.3295274111199686No Hit
CCTTTATTTAGCATGGCCATTGAATGTAACAATTATATATATCGCAAGCA257880.31739198020324755No Hit
CTTTAAACATGGACCTACGCTTAGCCTGCGCTTAGCCTACAACTTCTTCC254420.3131335024170554No Hit
GACTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAAT253960.31256734641079864No Hit
GCTTTTGTATTAGAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATA238320.2933180421980687No Hit
GCATGGCCATTGAATGTAACAATTATATATATCGCAAGCACAAAAAATCA218670.2691333345394918No Hit
GATATTGGAATTGGAAATGGCAATTCACTTCCATTTATTTCAGAAATAGT217490.26768102130605054No Hit
GGATTTATGGATCAAACAACTGTCTCGGACAAGGCAATTATTTTCAAATA215170.2648256257962338No Hit
GGGTACCCGTTTTATCGAAGACAACATGCGAAGTGTTGTGAGCAACTTCT211480.2602840700069132No Hit
CCCTGTAGTGTGGTAGCCTATTTAGTTCATTCTCAGTCAACTGTAATATC211280.2600379152215842No Hit
GTTTTATTTCCGTGCATATGCATCCGACTTGCAATTAGTCGAAAGATTTC199660.24573632219396777No Hit
CCCTGCAGGAATGGTCTACATTTTCTATCTACTAGTAAAGCTTTATTCAC187750.23107780472762418No Hit
GTTGAAAAAATCGAGGTGGAATTTGAGAACGACAATTACTTAGCACTACA187690.2310039582920255No Hit
CACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCT181480.22336085220755916No Hit
GGAGCGTTAACACACCATCCATGTGGCCCTTGAATTCCTGAATCCTGCAG180020.22156392227465732No Hit
AGTCCTTCCGGGCTATTTGACCGGACCAACTCTATAATCAGCCTTGATCC176560.21730544448846512No Hit
CACACCACACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAA175520.21602543960475418No Hit
GAACCTATAATTGCTTTTTGTTGAGGACTTATAGTGCTAGAAATGGCTTC173340.21334235244466782No Hit
GGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTA170360.2096746461432653No Hit
GGGTGTTTTATTCCTTCAGTTAATCCAAGCAACAAAGATTGAGAATTATG162270.19971768507670612No Hit
GTGATATATCTACGGTAGGAGACGGTACTGAAGACTGAGGAGGTGATGGT162260.1997053773374397No Hit
CACACCACACACCACACACTACCCCTAACACTACCCTATTCTAACCCTGA160920.19805614027573523No Hit
GTTCCTATGATGCCCCAGGCTCAAACTTCAGCAAGTATTCAGCCTCACGC156430.19252996534509856No Hit
GACCAACTCTATAATCAGCCTTGATCCGCTGAGTGAGACGCTAACATCAA155900.19187765516397662No Hit
GTGCCGGCTGCAATAATCTAGTCAATTTAGAATCACGATATGGAATGTGG154700.19040072645200246No Hit
CCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGTT151090.18595763257681353No Hit
CTCTTTTGTATAGGGCGATGAATTCTGCCTGGTTGACAGTGCCAAACCTG143960.1771822144798337No Hit
CGTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGC142410.17527451489353377No Hit
CTAACAAATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATT140940.1734652772213654No Hit
ACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCG139020.17110219128220674No Hit
CCTACAACTTCTTCCGCTCTCGAAAAGACCAATATAATAAAAAGTTATAA136350.16781602489806421No Hit
CCTTTTTCTTTGAGATAGGATGCTATTGCCATGGAAACTGGGTGTTTTAT134640.16571140148350105No Hit
GGTTTTGAAATCCATCTGGGATGGCCGCCAGTGAGATTATCTTATTAGGT131420.16174830943970372No Hit
GGATAATCAGCTCTGTCGTGACTGATAAATGCTATATCCGGCATATGCAG129970.15996368724606827No Hit
CCTCAGTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAA129590.1594959931539431No Hit
CCAATAAACTTGCAGACCAAGAGGTTGTTGACCTCATCACCTCTACGGTG126350.15550828563161287No Hit
GAACCATTAACACTAAAACAGAATACAGAATATCCTTGTTCAAGGGCTTT125720.15473289805782645No Hit
AACCCGTACTTCCAACTGCAGGACGACCCAACCATCAAAGACACACCAGA123330.15179134837314456No Hit
GCTTTTTGAAATCCATAAATCTTTCTATCCAATAAGCATATTAAAGAAGT121670.14974826365491364No Hit
GTTAAACTCCTTTACAATATCTGGACAGACGTAACAGTATTCTTGCTTAA121190.14915749217012397No Hit
CGTCTAAAGACATGGGGTCAATGCCTTTTTGGTTAATGATAACAAAACCC120030.1477297944152156No Hit
CTCAACCATCCACTCCCAACCACCATCCATCTCTCTACTTACCACTAACC118950.14640055857443887No Hit
GCATTATCAAGCATATCTACTGGAAACCAGAGGAAATTTGAGGTAAAACA118850.14627748118177436No Hit
GCTACAGGGCGCGTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGG116880.14385285654628344No Hit
TAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGT110630.13616051950475133No Hit
CTTAACTATCTGCTTAGTCGAGGAGAACCAGGATTCTGTTCGTTGCTCAG108190.1331574311237372No Hit
GTACTGTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAAT106960.13164357919396372No Hit
ATTTCCATGGTGCACAGTATCTTAACTATCTGCTTAGTCGAGGAGAACCA106000.13046203622438438No Hit
GTCCGACTAAACCGGACAAGAGTATCCTACTGCACATGATAACTTTAACT97740.12029584359029555No Hit
CAGTAATCACGCACTCCAAAATCTCCAAACAAATAAAATCGAAGCCTCAA96440.11869583748565686No Hit
GTCCAAGTATGTCATGAAAAGCCTCTTGCCATCAGTAGCACAACTAATCA94350.11612351997896853No Hit
GCTTTTGAATCGAGGGGTGGATCCAAAACCTGGGGTAGCCAGACTTTAAA93750.11538505562298144No Hit
CAAGAGTATCCTACTGCACATGATAACTTTAACTTTGGGATCTAATAAGT93330.1148681305737905No Hit
GGGTGGGTTCTCGGACCTGCTTTGGTCAGACCCTGACAATGTAGAGGCTT91720.11288658455189182No Hit
CCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTACCC90670.11159427192891443No Hit
CCTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTATT90230.11105273140119057No Hit
CCTTCTACCCGCTTACCAGTCACAGCTTTTGTATTAGAAACATTTTGAGC88240.10860349128716676No Hit
ATTATATATATCGCAAGCACAAAAAATCAAGGAGAGAGAACTACCACTTT88200.10855426033010095No Hit
GTTCTGGTATGACGCGTACTTGCTATATGATGCAACTGTCGTTCTCAACG88110.10844349067670289No Hit
CACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGAT87920.10820964363064031No Hit
CTATCCAGAATCATCATCGGAGCTTTTTGAAATCCATAAATCTTTCTATC87890.10817272041284097No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTTTG86620.10660963752600162No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTGGT86060.10592040412708036No Hit
CCATCCCGGTTGATCCTGAAAAGGTTGTTGCGATTGTGGAGTCCACCATG85520.105255786206692No Hit
AGAACTACCACTTTGTTCATGTGTACAATGTTCATTATCTCCATAAGCAA85240.10491116950723134No Hit
GTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGC84080.10348347175232299No Hit
GTTCCCCGTGAGTTGCTTTCTCTGAAAACCTTATTATACATTAGAGTTAT84030.10342193305599075No Hit
GAGGACCATCGAATTGGAACTGTCTGTTGGCAACGATAGAGCCAATTCTA83990.10337270209892493No Hit
ATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAACG82410.10142807929482561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGC196050.064.7801426
TCCGGGA64650.064.3196918
TCGTCTC196850.064.22246655
GCCGATT197150.064.1097446
GGACGAT75850.064.01134528
CGTCTCA198200.063.7661956
CCGATTG198600.063.62404647
CCGGGAC65550.063.5441619
TGCCGAT199750.063.18415545
CTCCATG202000.062.9312632
GTCATGG77650.062.9231614
GTGCCAG189400.062.826312
GCTCCAT201750.062.80144531
CGGGACT66450.062.63314420
TTATATG203000.062.3913566
CTCTCGT67100.062.1756556
TTGCCGA203950.062.1537444
TGTACAG204900.061.931325
TTCGTCT204650.061.8776954
TAGCAGT194250.061.57797227
Produced by FastQC (version 0.11.5)