FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n02_1744_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n02_1744_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10428410
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTAAATTTTCAACTAATGCATTGTACAGCTCCATGTTTATTTGCCGA2096002.0098941257583847No Hit
GCTTTGTACATCTGTCATGGCATTCTTGGACGATTCTGTATACTTCGGAA1407941.350100350868445No Hit
CCATAACCGTTACCCTCCAATTACCCATATCCAACTCCACTACCACTTAC1297241.2439480227570645No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTTGG1221831.1716359445016067No Hit
AGAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAA1159601.111962418048389No Hit
ATATTACAACGTCTCCAATGATATCAGCGGTTTTCAGAACAGAATGGGGC1104911.0595191405017639No Hit
GCCATACGTCCGTAGTATGGACCCTGCAGGAATGGTCTACATTTTCTATC931380.8931179345652884No Hit
GATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAAC861240.8258593591928204No Hit
CCCATATGCTGATCTTTTCCGGGACTTCTAATGACTAATGTTTTGTTGTA797440.7646803299831901No Hit
CGTTTGTTTTTAATTATATACAGGTTGGTTAGGTGACGTTGGAGAAAAGA732100.7020245655857412No Hit
GGATCAGATAAGTCTATCGGTTTGCAAATTTTACCTATTAGCGGTATCGG677810.6499648556203678No Hit
GAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAAG535280.5132901372308915No Hit
GTACCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTA483250.4633975840995895No Hit
GAATGGGGCTGTTCTTCTTTATACTAACGTACTTCGGTTTTGTTACATTC454940.43625058853650744No Hit
GGAGCGTTAACACACCATCCATGTGGCCCTTGAATTCCTGAATCCTGCAG454710.43603003717728783No Hit
GTTTTATTTCCGTGCATATGCATCCGACTTGCAATTAGTCGAAAGATTTC423310.40591998204903723No Hit
CCTCAGTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAA408620.39183346262757224No Hit
CTTTAAACATGGACCTACGCTTAGCCTGCGCTTAGCCTACAACTTCTTCC408600.3918142842485096No Hit
CTTTTATGACCGTACTTTTAGAGCATCCACTGGGGCCAGACAGTAGTAGG402380.38584980836004723No Hit
GTTCCTATGATGCCCCAGGCTCAAACTTCAGCAAGTATTCAGCCTCACGC388120.37217562408842764No Hit
GTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAATAATAC384750.36894406721638295No Hit
GCTTTTGTATTAGAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATA365930.35089721251849515No Hit
CCTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTATT337880.32399953588322666No Hit
CCCTGCAGGAATGGTCTACATTTTCTATCTACTAGTAAAGCTTTATTCAC312670.29982518907484457No Hit
CCTTTATTTAGCATGGCCATTGAATGTAACAATTATATATATCGCAAGCA308830.29614294029482924No Hit
GACTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAAT308350.2956826591973273No Hit
GAACTACCACTTTGTTCATGTGTACAATGTTCATTATCTCCATAAGCAAA295280.28314958847993127No Hit
GGATAATCAGCTCTGTCGTGACTGATAAATGCTATATCCGGCATATGCAG287300.2754974152339618No Hit
GGAACTGTCTGTTGGCAACGATAGAGCCAATTCTACCCTTCCTATCGGTT283910.2722466799828545No Hit
AACCCGTACTTCCAACTGCAGGACGACCCAACCATCAAAGACACACCAGA283530.2718822907806655No Hit
CTTAACTATCTGCTTAGTCGAGGAGAACCAGGATTCTGTTCGTTGCTCAG266690.2557340956099731No Hit
GCATTATCAAGCATATCTACTGGAAACCAGAGGAAATTTGAGGTAAAACA260570.24986551161682366No Hit
CCCTGTAGTGTGGTAGCCTATTTAGTTCATTCTCAGTCAACTGTAATATC259980.24929974943447755No Hit
GTTGAAAAAATCGAGGTGGAATTTGAGAACGACAATTACTTAGCACTACA256410.24587640877180703No Hit
AAATCAAGGAGAGAGAACTACCACTTTGTTCATGTGTACAATGTTCATTA256320.2457901060660254No Hit
GGTTTTGAAATCCATCTGGGATGGCCGCCAGTGAGATTATCTTATTAGGT255010.24453392223742643No Hit
GCATGGCCATTGAATGTAACAATTATATATATCGCAAGCACAAAAAATCA247800.23762011658536633No Hit
CCAATAAACTTGCAGACCAAGAGGTTGTTGACCTCATCACCTCTACGGTG236140.22643912159188215No Hit
CTAACAAATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATT235860.22617062428500603No Hit
AGTCCTTCCGGGCTATTTGACCGGACCAACTCTATAATCAGCCTTGATCC234590.22495279721453224No Hit
GACCAACTCTATAATCAGCCTTGATCCGCTGAGTGAGACGCTAACATCAA228970.21956367269794722No Hit
GGGTGTTTTATTCCTTCAGTTAATCCAAGCAACAAAGATTGAGAATTATG224180.21497045091245934No Hit
ATTTCCATGGTGCACAGTATCTTAACTATCTGCTTAGTCGAGGAGAACCA220940.21186355350432134No Hit
GCATATCAATGTACCAAAACAATAAAATGGTTTGAACCAAACATACTGTA219910.21087586698259853No Hit
CTCTTTTGTATAGGGCGATGAATTCTGCCTGGTTGACAGTGCCAAACCTG219370.21035805074790886No Hit
CCTTTTTCTTTGAGATAGGATGCTATTGCCATGGAAACTGGGTGTTTTAT216980.20806623444993055No Hit
CTCAACCATCCACTCCCAACCACCATCCATCTCTCTACTTACCACTAACC215200.2063593587133609No Hit
GGGTACCCGTTTTATCGAAGACAACATGCGAAGTGTTGTGAGCAACTTCT211670.20297437480881553No Hit
CCCTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTAT209920.20129626664083977No Hit
GTTCCCCGTGAGTTGCTTTCTCCGAAAACCTTATTATACATTAGAGTTAT203510.19514959615128288No Hit
GTTCCCCGTGAGTTGCTTTCTCTGAAAACCTTATTATACATTAGAGTTAT193640.18568506608389965No Hit
CACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCT181220.17377529268603747No Hit
GAAATTTGTGTTTACAAAGGCATTTAAAGAAGAATATGGCCTATGTGAGG176150.16891357359367343No Hit
GCTTTCCATCTGAGGAAGATCGTTGACTATACAATCACCTCTAAACTCGG175410.16820397356835798No Hit
GGAGGTGGTGTTTCCAACAGTCCCAACGCTTTCAAATGCGTGTCGTATGG172540.16545187617287774No Hit
ATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAACG172030.16496282750678196No Hit
GCTTCGAAAGGACGTTCCGTTGCTGTTTTGTCTGTATTTAGGAACTAAGA171130.16409980044896585No Hit
GCTTTTTGAAATCCATAAATCTTTCTATCCAATAAGCATATTAAAGAAGT167720.16082988681879595No Hit
CCTACAACTTCTTCCGCTCTCGAAAAGACCAATATAATAAAAAGTTATAA166300.15946822190535279No Hit
GATATTGGAATTGGAAATGGCAATTCACTTCCATTTATTTCAGAAATAGT163630.15690790830049833No Hit
GAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATAGGATGCTATTGC159970.15339826493204622No Hit
GAACCTATAATTGCTTTTTGTTGAGGACTTATAGTGCTAGAAATGGCTTC157580.1511064486340679No Hit
GTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAAGAATA152260.14600499980342163No Hit
GGATACTTCTCAATCAACTTTACAAAGTGATATGAACATTCCCCGCATGG147290.14123917260637048No Hit
GTCCAAGTATGTCATGAAAAGCCTCTTGCCATCAGTAGCACAACTAATCA146740.14071176718214953No Hit
GATTCTGCATTGAGCAAAGAATCATAGTTCCAGTCTAAGAAATCTTCCAT141910.13608018863853646No Hit
GTTCTGGTATGACGCGTACTTGCTATATGATGCAACTGTCGTTCTCAACG141460.1356486751096284No Hit
GGATTTATGGATCAAACAACTGTCTCGGACAAGGCAATTATTTTCAAATA141330.13552401564572164No Hit
GGATGCTATTGCCATGGAAACTGGGTGTTTTATTCCTTCAGTTAATCCAA140580.13480482643087488No Hit
ACCTTCTTTTCCCAGACGTCTAGGACCTCTTGTTCCTTTTGTATACCATG137810.13214862093070756No Hit
GTTTCTCTCTTTCTTCAGGAGTAGGCTCGTCCGGAAAACGGGCCAGCAGG135580.13001023166522988No Hit
GCTTTTGAATCGAGGGGTGGATCCAAAACCTGGGGTAGCCAGACTTTAAA135230.12967461003163475No Hit
ACATTTATTTTTTGAAAGAAAGGGGAAGATAGTTATATTAACACCAGAAT132950.1274882748185006No Hit
CTATCCAGAATCATCATCGGAGCTTTTTGAAATCCATAAATCTTTCTATC131570.1261649666631826No Hit
CTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTATTC129050.12374849090129753No Hit
ATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATTTTTTCAC125850.1206799502512847No Hit
GGATTCTGTTCGTTGCTCAGCCGCTTCGTGGATATTCTCTTGGATACTTT125320.12017172320612633No Hit
CCAATTGTTCCCCGTGAGTTGCTTTCTCTGAAAACCTTATTATACATTAG123660.11857991774393221No Hit
CACACCACACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAA122320.11729496634673933No Hit
GAAATATTATAAAAAACGTTCAAAAATATTTCGAATTTGGGTAGCTTCCA121700.11670043659579936No Hit
CCTTCTACCCGCTTACCAGTCACAGCTTTTGTATTAGAAACATTTTGAGC118830.11394833920031913No Hit
GTGATATATCTACGGTAGGAGACGGTACTGAAGACTGAGGAGGTGATGGT117310.11249078239156304No Hit
GACAAGATGAAAATATTTGTTAAGTTTCTGAACACGAAATTGTCGTATAT117240.11242365806484401No Hit
GAGGACCATCGAATTGGAACTGTCTGTTGGCAACGATAGAGCCAATTCTA115400.11065924719108665No Hit
ACAATAAAATGGTTTGAACCAAACATACTGTAATATGCCTTTTTTGACAA115270.11053458772717988No Hit
GTTAAACTCCTTTACAATATCTGGACAGACGTAACAGTATTCTTGCTTAA115250.1105154093481173No Hit
GATTTTATTTTTTTTTTCTTTTTTCGCGATGAGTTTTATTTCCGTGCATA114020.10933593903576865No Hit
GTGCCGGCTGCAATAATCTAGTCAATTTAGAATCACGATATGGAATGTGG111560.1069769984110713No Hit
GTATTCAGCCTCACGCGAACATTCAACCGCCTACTGGCATTTTACCTTTA110970.1064112362287252No Hit
GTCCACACGGCATTACCGTTTCACTAATTTATTGCCATCTTCCTCCACAG110090.10556738754997166No Hit
GCCCACCTAGAAGTGGAGGTTCTCGGAAGTGGTGGATTCTTCTTCTTGCG108680.10421531182605978No Hit
GGTTTTTGAATAATTTCGCCAACACTAGATCGTTTGGTGATGTCGCTGGG108570.10410983074121559No Hit
GGTCCCAATCGAAATTTGGAGGCTGAAACAATGCTATGTCTCTATTGCGC106210.10184678201183114No Hit
CCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTACCC105740.10139609010386053No Hit
CGTATATTGTCAAGGTATTATCAATACCAATGGTGGCAACATAAGATCTA105230.10090704143776472No Hit
CCATCCCGGTTGATCCTGAAAAGGTTGTTGCGATTGTGGAGTCCACCATG104800.10049470628791925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA229850.065.43262556
GGACGAT158550.065.35113528
GTACAGC232550.065.2084626
GCCGATT232450.064.7797646
TCGTCTC233200.064.6439955
CCGATTG232750.064.63611647
CGGGACT95250.064.6038920
CCGGGAC95850.064.26974519
GACGATT161900.064.0625429
GGAGCGT50200.064.0057141
GCTCCAT236900.063.900331
GTCATGG162550.063.86178214
GTCTCAA236050.063.7584857
CTCCATG237500.063.7385632
GAGCGTT50400.063.6737252
TCCGGGA97050.063.51112718
TGCCGAT237800.063.33494645
TGGACGA164900.063.0683927
GGCATTC164150.062.9023919
TTGCCGA241150.062.72637644
Produced by FastQC (version 0.11.5)