FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n02_1740_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n02_1740_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7954179
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTAAATTTTCAACTAATGCATTGTACAGCTCCATGTTTATTTGCCGA1885462.37040177245194No Hit
GCCATACGTCCGTAGTATGGACCCTGCAGGAATGGTCTACATTTTCTATC814061.0234368625599197No Hit
CCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGC658850.8283067303363426No Hit
GGAACTGTCTGTTGGCAACGATAGAGCCAATTCTACCCTTCCTATCGGTT601340.7560051137898707No Hit
GCTTTGTACATCTGTCATGGCATTCTTGGACGATTCTGTATACTTCGGAA587640.7387814631780351No Hit
GCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAT584560.7349092847923085No Hit
GTACCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTA558170.7017317563509697No Hit
CCCATATGCTGATCTTTTCCGGGACTTCTAATGACTAATGTTTTGTTGTA499080.6274437625806509No Hit
CCATAACCGTTACCCTCCAATTACCCATATCCAACTCCACTACCACTTAC407140.5118567233651644No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTTGG406390.5109138227842245No Hit
CTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGA400090.5029934579043294No Hit
CCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGTT377120.4741155561120764No Hit
CCCTGCAGGAATGGTCTACATTTTCTATCTACTAGTAAAGCTTTATTCAC319510.40168821948814576No Hit
AGAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAA316110.3974137368545515No Hit
ACATTTATTTTTTGAAAGAAAGGGGAAGATAGTTATATTAACACCAGAAT267960.3368795195582096No Hit
GGATTTATGGATCAAACAACTGTCTCGGACAAGGCAATTATTTTCAAATA263070.3307318077704814No Hit
CGTCTAAAGACATGGGGTCAATGCCTTTTTGGTTAATGATAACAAAACCC251100.31568311449868053No Hit
CGTTTGTTTTTAATTATATACAGGTTGGTTAGGTGACGTTGGAGAAAAGA235150.29563076214402517No Hit
GGAGCGTTAACACACCATCCATGTGGCCCTTGAATTCCTGAATCCTGCAG222080.2791991480201791No Hit
ATATTACAACGTCTCCAATGATATCAGCGGTTTTCAGAACAGAATGGGGC216870.2726491319845832No Hit
GACTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAAT216780.27253598391487044No Hit
GCTACAGGGCGCGTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGG186070.23392734812731775No Hit
CGTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGC182110.22894883305995506No Hit
CCAAGAGCTAAGTTACCTTCTGGGGTGATGGACCAACCAGCAGCATAGAT176660.22209708883845838No Hit
GTTTTATTTCCGTGCATATGCATCCGACTTGCAATTAGTCGAAAGATTTC174400.21925581508789282No Hit
GAGGACCATCGAATTGGAACTGTCTGTTGGCAACGATAGAGCCAATTCTA165410.2079535801243598No Hit
GGGTGGGTTCTCGGACCTGCTTTGGTCAGACCCTGACAATGTAGAGGCTT162010.20367909749076557No Hit
GCTTTTGTATTAGAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATA160800.20215788455351583No Hit
GAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAAG157580.19810969805934717No Hit
CCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCTGTCT157270.19771996581922535No Hit
GATATTGGAATTGGAAATGGCAATTCACTTCCATTTATTTCAGAAATAGT154220.19388550345673639No Hit
CTTTAAACATGGACCTACGCTTAGCCTGCGCTTAGCCTACAACTTCTTCC141600.17801962968145424No Hit
GTTGAAAAAATCGAGGTGGAATTTGAGAACGACAATTACTTAGCACTACA140960.17721502118571886No Hit
CAAGAGTATCCTACTGCACATGATAACTTTAACTTTGGGATCTAATAAGT140020.17603325245760748No Hit
CCTTTTTCTTTGAGATAGGATGCTATTGCCATGGAAACTGGGTGTTTTAT138930.17466290361330816No Hit
GGTGGGTTCTCGGACCTGCTTTGGTCAGACCCTGACAATGTAGAGGCTTG138140.1736697150013848No Hit
CCTTTATTTAGCATGGCCATTGAATGTAACAATTATATATATCGCAAGCA136940.17216107407188097No Hit
CACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCT132450.1665162425939874No Hit
GTCCGACTAAACCGGACAAGAGTATCCTACTGCACATGATAACTTTAACT132140.16612651035386555No Hit
GTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAATAATAC131550.16538476189685952No Hit
AGTCCTTCCGGGCTATTTGACCGGACCAACTCTATAATCAGCCTTGATCC131050.1647561615095662No Hit
GCATGGCCATTGAATGTAACAATTATATATATCGCAAGCACAAAAAATCA125740.1580804253965117No Hit
ACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCG125190.1573889649704891No Hit
CCATCCCGGTTGATCCTGAAAAGGTTGTTGCGATTGTGGAGTCCACCATG123270.15497513948328295No Hit
ACCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGT122960.1545854072431611No Hit
GACCAACTCTATAATCAGCCTTGATCCGCTGAGTGAGACGCTAACATCAA120910.1520081456552587No Hit
GAATGGGGCTGTTCTTCTTTATACTAACGTACTTCGGTTTTGTTACATTC116820.14686619448719976No Hit
GTCCCTACGGCCTTGTCTAACACCATCCAGCATGCAATACAGTGACATAT115240.14487981726335303No Hit
AACCCGTACTTCCAACTGCAGGACGACCCAACCATCAAAGACACACCAGA113700.14294372807048975No Hit
GGGTGTTTTATTCCTTCAGTTAATCCAAGCAACAAAGATTGAGAATTATG113670.14290601204725215No Hit
GCTCTTTTACATTCGAGCTTGATCTTTTTGTAATCATGCAACGTTCTCAT111390.14003959428119483No Hit
GAACCATTAACACTAAAACAGAATACAGAATATCCTTGTTCAAGGGCTTT110150.13848066532070752No Hit
GCTTCAAAGCCTTGACAAGACCTCTTGGGACCCAAGTTCTAATTCTGAAG109090.1371480324996458No Hit
CACACCACACACCACACACTACCCCTAACACTACCCTATTCTAACCCTGA108330.13619255991096002No Hit
CCCTGTAGTGTGGTAGCCTATTTAGTTCATTCTCAGTCAACTGTAATATC105380.1324838176259297No Hit
CTTTTATGACCGTACTTTTAGAGCATCCACTGGGGCCAGACAGTAGTAGG103580.13022085623167395No Hit
CTCTTTTGTATAGGGCGATGAATTCTGCCTGGTTGACAGTGCCAAACCTG103470.13008256414646943No Hit
GGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTA100180.1259463735980797No Hit
GATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAAC99640.12526748517980296No Hit
GCATTATCAAGCATATCTACTGGAAACCAGAGGAAATTTGAGGTAAAACA97700.12282851567710508No Hit
GTTCTGGTATGACGCGTACTTGCTATATGATGCAACTGTCGTTCTCAACG97440.12250164347571257No Hit
CTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTG96360.12114386663915912No Hit
TAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGT95890.12055298227510343No Hit
CTCAACCATCCACTCCCAACCACCATCCATCTCTCTACTTACCACTAACC90460.11372638206909852No Hit
CACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGAT88180.11085996430304122No Hit
CTAACAAATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATT87530.11004278379955995No Hit
CTCTTCGCGGTTCTGCAGATCATGAATCGGACCAGGAAAACCATACTTGA86330.1085341428700561No Hit
CCAATTGTTCCCCGTGAGTTGCTTTCTCTGAAAACCTTATTATACATTAG85410.10737751815743649No Hit
GTGATATATCTACGGTAGGAGACGGTACTGAAGACTGAGGAGGTGATGGT84480.10620832143707101No Hit
GTTCCTATGATGCCCCAGGCTCAAACTTCAGCAAGTATTCAGCCTCACGC82520.10374420791888139No Hit
GATAGCACCAGCTTGTGGTGGAGGACCATCGAATTGGAACTGTCTGTTGG82490.1037064918956438No Hit
CCAATAAACTTGCAGACCAAGAGGTTGTTGACCTCATCACCTCTACGGTG81300.1022104229738858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCTC210250.063.7586555
CGTCTCA210750.063.6057956
CCGATTG210450.063.6034747
CCGGGAC56650.063.45302619
GCCGATT211000.063.42108546
GTACAGC212550.063.3742926
CGGGACT56950.063.3055420
TGCCGAT211750.063.25860245
TCCGGGA57850.062.43931618
TTGCCGA217150.061.9210644
CTCCATG217050.061.89053732
TGTACAG219250.061.43765625
GGTTTAA223100.061.198261
TTCGTCT219800.061.11543354
GCTCCAT220100.061.0014831
TTATATG221750.060.76129566
CTCAACT221700.060.6194359
CTCTCGT58900.060.43257556
GTCTCAA223150.060.1497657
ATACGTC103850.059.917714
Produced by FastQC (version 0.11.5)