FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n02_1736_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n02_1736_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8696712
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTAAATTTTCAACTAATGCATTGTACAGCTCCATGTTTATTTGCCGA1969712.2648904551513263No Hit
CCATAACCGTTACCCTCCAATTACCCATATCCAACTCCACTACCACTTAC1209351.3905830157420414No Hit
GTACCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTA957871.101416259386306No Hit
GCTTTGTACATCTGTCATGGCATTCTTGGACGATTCTGTATACTTCGGAA914281.0512938683033313No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTTGG816770.9391710338343964No Hit
GCCATACGTCCGTAGTATGGACCCTGCAGGAATGGTCTACATTTTCTATC774270.8903019899934596No Hit
AGAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAA699900.8047869125710958No Hit
CCCATATGCTGATCTTTTCCGGGACTTCTAATGACTAATGTTTTGTTGTA620810.7138444966327504No Hit
ATATTACAACGTCTCCAATGATATCAGCGGTTTTCAGAACAGAATGGGGC497760.5723542414650502No Hit
CGTTTGTTTTTAATTATATACAGGTTGGTTAGGTGACGTTGGAGAAAAGA497070.5715608381650444No Hit
CCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGC459210.528027144051683No Hit
ACATTTATTTTTTGAAAGAAAGGGGAAGATAGTTATATTAACACCAGAAT402290.46257712110048027No Hit
GTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAATAATAC400090.4600474294193023No Hit
GACTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAAT387990.44613412517282397No Hit
CTCAACCATCCACTCCCAACCACCATCCATCTCTCTACTTACCACTAACC355670.4089706546566104No Hit
GATATTGGAATTGGAAATGGCAATTCACTTCCATTTATTTCAGAAATAGT340000.39095235072749335No Hit
GATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAAC323380.3718416799360494No Hit
CACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCT307120.35314495869243456No Hit
GAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAAG302560.34790159775326585No Hit
GGAGCGTTAACACACCATCCATGTGGCCCTTGAATTCCTGAATCCTGCAG298670.34342864291700126No Hit
GTTCCTATGATGCCCCAGGCTCAAACTTCAGCAAGTATTCAGCCTCACGC278930.3207304093777051No Hit
GGATCAGATAAGTCTATCGGTTTGCAAATTTTACCTATTAGCGGTATCGG276920.3184191910689925No Hit
GCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAT271360.3120259702747429No Hit
GTTGAAAAAATCGAGGTGGAATTTGAGAACGACAATTACTTAGCACTACA262820.3022061671123523No Hit
GGATTTATGGATCAAACAACTGTCTCGGACAAGGCAATTATTTTCAAATA260770.2998489544094366No Hit
GCTTTTGTATTAGAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATA247860.28500426368034265No Hit
GTGATATATCTACGGTAGGAGACGGTACTGAAGACTGAGGAGGTGATGGT234770.26995259817733414No Hit
GTTTTATTTCCGTGCATATGCATCCGACTTGCAATTAGTCGAAAGATTTC231280.26593958728310196No Hit
CTTTAAACATGGACCTACGCTTAGCCTGCGCTTAGCCTACAACTTCTTCC214780.24696689967426771No Hit
CCTTTATTTAGCATGGCCATTGAATGTAACAATTATATATATCGCAAGCA211120.24275841260467174No Hit
GGATAATCAGCTCTGTCGTGACTGATAAATGCTATATCCGGCATATGCAG209840.24128659199016822No Hit
GCATATCAATGTACCAAAACAATAAAATGGTTTGAACCAAACATACTGTA203420.2339044917205491No Hit
CCCTGCAGGAATGGTCTACATTTTCTATCTACTAGTAAAGCTTTATTCAC202670.23304209682923843No Hit
CTTTTATGACCGTACTTTTAGAGCATCCACTGGGGCCAGACAGTAGTAGG196100.2254875175813572No Hit
AGTCCTTCCGGGCTATTTGACCGGACCAACTCTATAATCAGCCTTGATCC195360.22463662128859732No Hit
CGTCTAAAGACATGGGGTCAATGCCTTTTTGGTTAATGATAACAAAACCC193190.22214142540307188No Hit
CTCTTTTGTATAGGGCGATGAATTCTGCCTGGTTGACAGTGCCAAACCTG191110.21974971690450368No Hit
CACACCACACACCACACACTACCCCTAACACTACCCTATTCTAACCCTGA189330.21770296636245975No Hit
GAACTACCACTTTGTTCATGTGTACAATGTTCATTATCTCCATAAGCAAA187320.21539174805374722No Hit
CACACCACACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAA181280.2084465945290588No Hit
GGAACTGTCTGTTGGCAACGATAGAGCCAATTCTACCCTTCCTATCGGTT181250.20841209873340635No Hit
ATTTCCATGGTGCACAGTATCTTAACTATCTGCTTAGTCGAGGAGAACCA173150.19909823390725137No Hit
CCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGTT170210.19571764593331364No Hit
GAACCATTAACACTAAAACAGAATACAGAATATCCTTGTTCAAGGGCTTT170130.19562565714490715No Hit
CCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTACCC166640.19161264625067498No Hit
AACCCGTACTTCCAACTGCAGGACGACCCAACCATCAAAGACACACCAGA165610.19042829059994168No Hit
CCCTGTAGTGTGGTAGCCTATTTAGTTCATTCTCAGTCAACTGTAATATC163940.18850802464195662No Hit
GGTTTTGAAATCCATCTGGGATGGCCGCCAGTGAGATTATCTTATTAGGT156990.18051649864914465No Hit
GTTAAACTCCTTTACAATATCTGGACAGACGTAACAGTATTCTTGCTTAA153870.17692893590129233No Hit
CTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGA150660.17323788576648277No Hit
GAAATATTATAAAAAACGTTCAAAAATATTTCGAATTTGGGTAGCTTCCA147420.1695123398360208No Hit
CCTTTTTCTTTGAGATAGGATGCTATTGCCATGGAAACTGGGTGTTTTAT146750.1687419337331166No Hit
AAATCAAGGAGAGAGAACTACCACTTTGTTCATGTGTACAATGTTCATTA145950.16782204584905192No Hit
GAATGGGGCTGTTCTTCTTTATACTAACGTACTTCGGTTTTGTTACATTC145920.16778755005339951No Hit
GACCAACTCTATAATCAGCCTTGATCCGCTGAGTGAGACGCTAACATCAA145520.16732760611136716No Hit
GCATGGCCATTGAATGTAACAATTATATATATCGCAAGCACAAAAAATCA141510.16271666809249288No Hit
CTAACAAATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATT139300.1601754778127642No Hit
GTTCTGGTATGACGCGTACTTGCTATATGATGCAACTGTCGTTCTCAACG135670.15600148653882065No Hit
GCATTATCAAGCATATCTACTGGAAACCAGAGGAAATTTGAGGTAAAACA135500.1558060103634569No Hit
CCTCAGTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAA132760.15265539436053532No Hit
CTTAACTATCTGCTTAGTCGAGGAGAACCAGGATTCTGTTCGTTGCTCAG132650.15252890977647643No Hit
CAGTAATCACGCACTCCAAAATCTCCAAACAAATAAAATCGAAGCCTCAA126690.1456757450401945No Hit
CACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGAT124210.14282409259959394No Hit
GGGTGTTTTATTCCTTCAGTTAATCCAAGCAACAAAGATTGAGAATTATG118340.13607441525026928No Hit
GGATACTTCTCAATCAACTTTACAAAGTGATATGAACATTCCCCGCATGG118240.1359594292647612No Hit
GAACCTATAATTGCTTTTTGTTGAGGACTTATAGTGCTAGAAATGGCTTC118240.1359594292647612No Hit
GGGTGGGTTCTCGGACCTGCTTTGGTCAGACCCTGACAATGTAGAGGCTT117550.1351660259647554No Hit
CCTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTATT114180.1312909982531329No Hit
GGGTACCCGTTTTATCGAAGACAACATGCGAAGTGTTGTGAGCAACTTCT110360.126898533606724No Hit
GTCCGACTAAACCGGACAAGAGTATCCTACTGCACATGATAACTTTAACT108460.12471379988207038No Hit
CAAGAGTATCCTACTGCACATGATAACTTTAACTTTGGGATCTAATAAGT104210.1198268954979767No Hit
GCTTTTGAATCGAGGGGTGGATCCAAAACCTGGGGTAGCCAGACTTTAAA102620.11799861832839814No Hit
CCATCCCGGTTGATCCTGAAAAGGTTGTTGCGATTGTGGAGTCCACCATG102470.11782613935013601No Hit
GAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATAGGATGCTATTGC102260.11758466878056902No Hit
GAGTTGAGCTGATAGTTCTTCCCCCTCTAGCCATCGATGTCAACAATAAC99380.11427307239793615No Hit
CCTACAACTTCTTCCGCTCTCGAAAAGACCAATATAATAAAAAGTTATAA99100.1139511116385135No Hit
CCTTCTACCCGCTTACCAGTCACAGCTTTTGTATTAGAAACATTTTGAGC94480.10863875910803991No Hit
CCCCTTATTTCAACGTTATGGATCCCAGATAAAATACATTACTCCAAGAA91990.10577560806888857No Hit
CCTTTTCTCTTCTACTTAGTTTCCTTCTTAAAATTTCAGAGTTTGTCTTG91310.10499370336743358No Hit
CTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAATAT90660.10424629446163101No Hit
GCTTTCCATCTGAGGAAGATCGTTGACTATACAATCACCTCTAAACTCGG89450.10285496403698317No Hit
GTTCCCCGTGAGTTGCTTTCTCTGAAAACCTTATTATACATTAGAGTTAT88160.10137164482392885No Hit
GTCCACACGGCATTACCGTTTCACTAATTTATTGCCATCTTCCTCCACAG87740.1008887036847949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAGC216750.065.5546426
TCGTCTC216450.064.9723255
CGTCTCA216800.064.94591556
CCGATTG217700.064.890947
GCCGATT218050.064.7225346
TGCCGAT218800.064.57732445
GGACGAT103700.064.5757928
CTCCATG218700.064.42969532
GCTCCAT219850.064.1570931
CCGGGAC68850.064.1057819
CGGGACT69250.063.78897520
GTCATGG106150.063.17519414
TTGCCGA225800.062.99025744
TTGGACG107900.062.74491526
ATACGTC91100.062.6573034
TCCGGGA71000.062.65717318
GGAGCGT36050.062.5257871
TTCGTCT226000.062.1958454
TGTACAG228050.062.0304325
CGTCCGT91250.062.0173577
Produced by FastQC (version 0.11.5)