FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n02_1734_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n02_1734_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11449328
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGC4032453.521997098869034No Hit
GCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAT2195611.917675867090191No Hit
GGTTTAAATTTTCAACTAATGCATTGTACAGCTCCATGTTTATTTGCCGA1658021.4481373928670749No Hit
CTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGA1044600.9123679573159228No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTTGG973690.8504341914215402No Hit
GCCATACGTCCGTAGTATGGACCCTGCAGGAATGGTCTACATTTTCTATC869160.759136256730526No Hit
ATATTACAACGTCTCCAATGATATCAGCGGTTTTCAGAACAGAATGGGGC867850.7579920847756305No Hit
GCTTTGTACATCTGTCATGGCATTCTTGGACGATTCTGTATACTTCGGAA816230.7129064692705108No Hit
AGAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAA801860.7003555143149013No Hit
CGTTTGTTTTTAATTATATACAGGTTGGTTAGGTGACGTTGGAGAAAAGA770610.6730613360015539No Hit
CCATAACCGTTACCCTCCAATTACCCATATCCAACTCCACTACCACTTAC754370.6588770974156737No Hit
CCCATATGCTGATCTTTTCCGGGACTTCTAATGACTAATGTTTTGTTGTA726130.634211894357468No Hit
GTACCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTA645660.5639282934334662No Hit
GATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAAC623790.5448267356826532No Hit
ACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCG597930.522240257244792No Hit
GGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTA533450.4659225414801637No Hit
GTTGAAAAAATCGAGGTGGAATTTGAGAACGACAATTACTTAGCACTACA478080.41756162457744245No Hit
CGTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGC473400.4134740484332356No Hit
GGAGCGTTAACACACCATCCATGTGGCCCTTGAATTCCTGAATCCTGCAG449410.3925208536256451No Hit
GCTACAGGGCGCGTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGG382680.3342379570224558No Hit
AACCCGTACTTCCAACTGCAGGACGACCCAACCATCAAAGACACACCAGA361190.31546829647993313No Hit
GGATCAGATAAGTCTATCGGTTTGCAAATTTTACCTATTAGCGGTATCGG348440.30433227172808747No Hit
GATATTGGAATTGGAAATGGCAATTCACTTCCATTTATTTCAGAAATAGT347090.30315316322495084No Hit
GCTTTTGTATTAGAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATA338700.29582522223138336No Hit
ACATTTATTTTTTGAAAGAAAGGGGAAGATAGTTATATTAACACCAGAAT322890.28201655153909466No Hit
GAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAAG321360.2806802285688732No Hit
CTTTTATGACCGTACTTTTAGAGCATCCACTGGGGCCAGACAGTAGTAGG306280.2675091498819844No Hit
GAATGGGGCTGTTCTTCTTTATACTAACGTACTTCGGTTTTGTTACATTC301760.2635613199307418No Hit
CCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGTT294870.25754349949621497No Hit
CCTCAGTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAA283660.24775253185165105No Hit
GTTTTATTTCCGTGCATATGCATCCGACTTGCAATTAGTCGAAAGATTTC278630.24335926091033463No Hit
GTTCTGGTATGACGCGTACTTGCTATATGATGCAACTGTCGTTCTCAACG269640.23550727169315092No Hit
GGATTTATGGATCAAACAACTGTCTCGGACAAGGCAATTATTTTCAAATA269520.23540246204842766No Hit
GACTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAAT267840.2339351270223021No Hit
CTTTAAACATGGACCTACGCTTAGCCTGCGCTTAGCCTACAACTTCTTCC267250.2334198129357461No Hit
GGAACTGTCTGTTGGCAACGATAGAGCCAATTCTACCCTTCCTATCGGTT265500.23189133895019864No Hit
GTTCCTATGATGCCCCAGGCTCAAACTTCAGCAAGTATTCAGCCTCACGC263770.23038033323877172No Hit
GTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAATAATAC258840.2260744036680581No Hit
CACACCACACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAA257800.22516605341378987No Hit
CACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCT255200.2228951777781194No Hit
GCATTATCAAGCATATCTACTGGAAACCAGAGGAAATTTGAGGTAAAACA241070.21055384211195624No Hit
CTAACAAATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATT234630.20492905784514165No Hit
GTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCG225430.19689365174969223No Hit
CCCTGCAGGAATGGTCTACATTTTCTATCTACTAGTAAAGCTTTATTCAC219840.19201126913300062No Hit
GTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCG218480.19082342649280376No Hit
CCTTTATTTAGCATGGCCATTGAATGTAACAATTATATATATCGCAAGCA215580.18829052674532515No Hit
GTGATATATCTACGGTAGGAGACGGTACTGAAGACTGAGGAGGTGATGGT211650.18485801088063858No Hit
GAACCATTAACACTAAAACAGAATACAGAATATCCTTGTTCAAGGGCTTT206970.18077043473643167No Hit
CCTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTATT206810.18063068854346737No Hit
AGTCCTTCCGGGCTATTTGACCGGACCAACTCTATAATCAGCCTTGATCC206560.18041233511696056No Hit
CGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATG202720.17705842648581646No Hit
GGATAATCAGCTCTGTCGTGACTGATAAATGCTATATCCGGCATATGCAG201840.1762898224245126No Hit
GGTTTTGAAATCCATCTGGGATGGCCGCCAGTGAGATTATCTTATTAGGT199730.1744469195047954No Hit
GCATATCAATGTACCAAAACAATAAAATGGTTTGAACCAAACATACTGTA197150.17219351214324544No Hit
CCAATAAACTTGCAGACCAAGAGGTTGTTGACCTCATCACCTCTACGGTG195790.17100566950304855No Hit
CCTTTTTCTTTGAGATAGGATGCTATTGCCATGGAAACTGGGTGTTTTAT192650.16826315046612342No Hit
GGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGA184140.1608303998278327No Hit
CTCTTTTGTATAGGGCGATGAATTCTGCCTGGTTGACAGTGCCAAACCTG183820.160550907441904No Hit
CACACCACACACCACACACTACCCCTAACACTACCCTATTCTAACCCTGA179160.15648079957181765No Hit
GTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGC177540.15506586936805375No Hit
ATTTCCATGGTGCACAGTATCTTAACTATCTGCTTAGTCGAGGAGAACCA177370.15491738903802912No Hit
GTTAAACTCCTTTACAATATCTGGACAGACGTAACAGTATTCTTGCTTAA176670.15430599944381015No Hit
GAAATATTATAAAAAACGTTCAAAAATATTTCGAATTTGGGTAGCTTCCA176550.1542011897990869No Hit
CGTCTAAAGACATGGGGTCAATGCCTTTTTGGTTAATGATAACAAAACCC171450.14974677989834864No Hit
GGGTGTTTTATTCCTTCAGTTAATCCAAGCAACAAAGATTGAGAATTATG168850.14747590426267812No Hit
CCGTAGGAAGAACATTTCTTGATCACAGACTCAATTGCATTGTAACCTCT162950.14232276339711816No Hit
TCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGG157510.13757139283633066No Hit
GAACTACCACTTTGTTCATGTGTACAATGTTCATTATCTCCATAAGCAAA157510.13757139283633066No Hit
GCTTTCCATCTGAGGAAGATCGTTGACTATACAATCACCTCTAAACTCGG157040.13716088839449792No Hit
CCCTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTAT154810.13521317583005746No Hit
GGTTTTTGAATAATTTCGCCAACACTAGATCGTTTGGTGATGTCGCTGGG153850.13437469867227142No Hit
CTTAACTATCTGCTTAGTCGAGGAGAACCAGGATTCTGTTCGTTGCTCAG153240.1338419163115949No Hit
AATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCT150760.13167585032064763No Hit
GACCAACTCTATAATCAGCCTTGATCCGCTGAGTGAGACGCTAACATCAA149340.13043560285808914No Hit
CAAGAGTATCCTACTGCACATGATAACTTTAACTTTGGGATCTAATAAGT145220.12683713838925742No Hit
GGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCA143850.1256405616120003No Hit
GCATGGCCATTGAATGTAACAATTATATATATCGCAAGCACAAAAAATCA143170.12504664029190185No Hit
ATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCAC142120.12412955590057337No Hit
CACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGAT141840.1238850000628858No Hit
CCCTGTAGTGTGGTAGCCTATTTAGTTCATTCTCAGTCAACTGTAATATC141050.12319500323512438No Hit
ATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAACG134830.11776236998363573No Hit
CACACACCACACCCACACCCACACACCCTAACACAATCCTAACAGTACCC134650.11760515551655085No Hit
GTCCGACTAAACCGGACAAGAGTATCCTACTGCACATGATAACTTTAACT134390.11737806795298379No Hit
ATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATTTTTTCAC132130.1154041529773625No Hit
GCTCTTTTACATTCGAGCTTGATCTTTTTGTAATCATGCAACGTTCTCAT131710.11503731922083112No Hit
AAATCAAGGAGAGAGAACTACCACTTTGTTCATGTGTACAATGTTCATTA130130.11365732556530828No Hit
GAACCTATAATTGCTTTTTGTTGAGGACTTATAGTGCTAGAAATGGCTTC129820.11338656731643987No Hit
GCTTCGAAAGGACGTTCCGTTGCTGTTTTGTCTGTATTTAGGAACTAAGA128390.11213758571682111No Hit
CCTTCTACCCGCTTACCAGTCACAGCTTTTGTATTAGAAACATTTTGAGC127460.11132531097021589No Hit
GGGTACCCGTTTTATCGAAGACAACATGCGAAGTGTTGTGAGCAACTTCT125460.10957848355816167No Hit
CCATCCCGGTTGATCCTGAAAAGGTTGTTGCGATTGTGGAGTCCACCATG123970.10827709713618126No Hit
GCTTTTGAATCGAGGGGTGGATCCAAAACCTGGGGTAGCCAGACTTTAAA123350.10773558063844446No Hit
GGTCCCAATCGAAATTTGGAGGCTGAAACAATGCTATGTCTCTATTGCGC122650.10712419104422546No Hit
CTCAACCATCCACTCCCAACCACCATCCATCTCTCTACTTACCACTAACC122040.10659140868354894No Hit
GTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAAGAATA120000.10480964472325363No Hit
GCGCGCGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCC117760.1028531980217529No Hit
GAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATAGGATGCTATTGC116870.10207585982338876No Hit
CAGTAATCACGCACTCCAAAATCTCCAAACAAATAAAATCGAAGCCTCAA116490.10174396261509845No Hit
CCTACAACTTCTTCCGCTCTCGAAAAGACCAATATAATAAAAAGTTATAA115420.10080940994964946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA184250.065.64870556
GGACGAT92750.065.2165928
GCCGATT186700.064.99853546
CCGATTG187450.064.8691947
TCGTCTC186650.064.8258855
TGCCGAT187850.064.5972445
GTACAGC189150.064.47713526
TCCGGGA82400.063.71685818
CCGGGAC82350.063.71331819
GCTCCAT191650.063.57237231
CTCCATG191900.063.5431632
CGGGACT82800.063.49691820
TTGCCGA194700.062.41062544
ATACGTC103400.062.2441254
GTCCGTA103050.062.218348
TTCGTCT195200.062.0940154
TCCGTAG103100.062.0548259
CGTCCGT103350.061.9358757
CCATACG104150.061.7617572
GGCATTC98450.061.6489319
Produced by FastQC (version 0.11.5)