FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n02_1728_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n02_1728_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7571960
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTAAATTTTCAACTAATGCATTGTACAGCTCCATGTTTATTTGCCGA2357943.1140418068769513No Hit
CCATAACCGTTACCCTCCAATTACCCATATCCAACTCCACTACCACTTAC1041521.3754959085890575No Hit
GCCATACGTCCGTAGTATGGACCCTGCAGGAATGGTCTACATTTTCTATC920991.2163165151427107No Hit
GCTTTGTACATCTGTCATGGCATTCTTGGACGATTCTGTATACTTCGGAA852671.1260888858366922No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTTGG727020.9601477028404798No Hit
GTACCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTA705200.9313308575322637No Hit
AGAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAA617260.8151918393652371No Hit
GGAACTGTCTGTTGGCAACGATAGAGCCAATTCTACCCTTCCTATCGGTT589880.7790321132177137No Hit
CCCATATGCTGATCTTTTCCGGGACTTCTAATGACTAATGTTTTGTTGTA481500.6358987633320831No Hit
ATATTACAACGTCTCCAATGATATCAGCGGTTTTCAGAACAGAATGGGGC416030.5494350207872203No Hit
CCCTGCAGGAATGGTCTACATTTTCTATCTACTAGTAAAGCTTTATTCAC310460.41001273118188686No Hit
ACATTTATTTTTTGAAAGAAAGGGGAAGATAGTTATATTAACACCAGAAT300090.3963174660193662No Hit
CGTTTGTTTTTAATTATATACAGGTTGGTTAGGTGACGTTGGAGAAAAGA298730.39452136567018314No Hit
GTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAATAATAC296680.391814008526194No Hit
CCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGTT266160.3515074036312923No Hit
GAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAAG264880.3498169562438259No Hit
GACTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAAT261110.3448380604229288No Hit
GGAGCGTTAACACACCATCCATGTGGCCCTTGAATTCCTGAATCCTGCAG239100.31577028933063567No Hit
GGATTTATGGATCAAACAACTGTCTCGGACAAGGCAATTATTTTCAAATA226620.2992884273028384No Hit
GATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAAC223310.29491703601181196No Hit
AGTCCTTCCGGGCTATTTGACCGGACCAACTCTATAATCAGCCTTGATCC221840.29297566284026855No Hit
GATATTGGAATTGGAAATGGCAATTCACTTCCATTTATTTCAGAAATAGT220760.2915493478570938No Hit
GTTCCTATGATGCCCCAGGCTCAAACTTCAGCAAGTATTCAGCCTCACGC220450.2911399426304418No Hit
CCTTTATTTAGCATGGCCATTGAATGTAACAATTATATATATCGCAAGCA209620.2768371729380504No Hit
CGTCTAAAGACATGGGGTCAATGCCTTTTTGGTTAATGATAACAAAACCC204500.2700753833881848No Hit
CTTTAAACATGGACCTACGCTTAGCCTGCGCTTAGCCTACAACTTCTTCC200480.2647663220619232No Hit
CACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCT199220.263102287914886No Hit
GCTTTTGTATTAGAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATA198200.2617552126529987No Hit
GTTTTATTTCCGTGCATATGCATCCGACTTGCAATTAGTCGAAAGATTTC195420.2580837722333451No Hit
CTCAACCATCCACTCCCAACCACCATCCATCTCTCTACTTACCACTAACC184380.2435036635164475No Hit
GAGGACCATCGAATTGGAACTGTCTGTTGGCAACGATAGAGCCAATTCTA182690.2412717447001833No Hit
GCATGGCCATTGAATGTAACAATTATATATATCGCAAGCACAAAAAATCA182340.24080951299267297No Hit
GTTGAAAAAATCGAGGTGGAATTTGAGAACGACAATTACTTAGCACTACA178810.23614757605692582No Hit
CTTTTATGACCGTACTTTTAGAGCATCCACTGGGGCCAGACAGTAGTAGG176220.23272706142134927No Hit
GACCAACTCTATAATCAGCCTTGATCCGCTGAGTGAGACGCTAACATCAA168670.22275606315934052No Hit
CCCTGTAGTGTGGTAGCCTATTTAGTTCATTCTCAGTCAACTGTAATATC166130.2194015816248369No Hit
GAATGGGGCTGTTCTTCTTTATACTAACGTACTTCGGTTTTGTTACATTC151660.20029160217433797No Hit
AACCCGTACTTCCAACTGCAGGACGACCCAACCATCAAAGACACACCAGA141110.18635861784795482No Hit
CTCTTTTGTATAGGGCGATGAATTCTGCCTGGTTGACAGTGCCAAACCTG137820.18201363979735763No Hit
CCTTTTTCTTTGAGATAGGATGCTATTGCCATGGAAACTGGGTGTTTTAT133730.17661213212959392No Hit
AAATCAAGGAGAGAGAACTACCACTTTGTTCATGTGTACAATGTTCATTA131990.17431418021225678No Hit
GGATCAGATAAGTCTATCGGTTTGCAAATTTTACCTATTAGCGGTATCGG130520.17237280704071337No Hit
GAACCATTAACACTAAAACAGAATACAGAATATCCTTGTTCAAGGGCTTT130320.17210867463642177No Hit
CACACCACACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAA128080.16915039170835555No Hit
CACACCACACACCACACACTACCCCTAACACTACCCTATTCTAACCCTGA125370.1655713976302041No Hit
GAACTACCACTTTGTTCATGTGTACAATGTTCATTATCTCCATAAGCAAA123140.16262632132235247No Hit
ATTTCCATGGTGCACAGTATCTTAACTATCTGCTTAGTCGAGGAGAACCA121650.16065853491037987No Hit
GGATAATCAGCTCTGTCGTGACTGATAAATGCTATATCCGGCATATGCAG121500.16046043560716117No Hit
GGGTGTTTTATTCCTTCAGTTAATCCAAGCAACAAAGATTGAGAATTATG120100.1586115087771198No Hit
GGGTGGGTTCTCGGACCTGCTTTGGTCAGACCCTGACAATGTAGAGGCTT118450.1564324164417139No Hit
GTGATATATCTACGGTAGGAGACGGTACTGAAGACTGAGGAGGTGATGGT118420.15639279658107014No Hit
GGTTTTGAAATCCATCTGGGATGGCCGCCAGTGAGATTATCTTATTAGGT113540.14994796591635456No Hit
GTTAAACTCCTTTACAATATCTGGACAGACGTAACAGTATTCTTGCTTAA113180.14947252758862964No Hit
CCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTACCC112650.14877257671725683No Hit
CCATCCCGGTTGATCCTGAAAAGGTTGTTGCGATTGTGGAGTCCACCATG107760.14231453943232664No Hit
CAAGAGTATCCTACTGCACATGATAACTTTAACTTTGGGATCTAATAAGT107250.141641001801383No Hit
GCTTTTGAATCGAGGGGTGGATCCAAAACCTGGGGTAGCCAGACTTTAAA104740.13832614012752315No Hit
CTAACAAATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATT103120.13618666765276097No Hit
GCATTATCAAGCATATCTACTGGAAACCAGAGGAAATTTGAGGTAAAACA101090.133505723749201No Hit
GTTCTGGTATGACGCGTACTTGCTATATGATGCAACTGTCGTTCTCAACG100930.1332944178257677No Hit
CCTCAGTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAA100910.13326800458533855No Hit
CCAATAAACTTGCAGACCAAGAGGTTGTTGACCTCATCACCTCTACGGTG98210.12970221712740163No Hit
AGATGAAGCGGTGGCTCAATGGTAGAGCTTTCGACTCCAATTAAATCTTG97460.12871172061130803No Hit
CGATAGAGCCAATTCTACCCTTCCTATCGGTTAAGATACCCTTGCTTAAA96450.12737785196963533No Hit
GTCCGACTAAACCGGACAAGAGTATCCTACTGCACATGATAACTTTAACT95480.126096809808821No Hit
CTATCCAGAATCATCATCGGAGCTTTTTGAAATCCATAAATCTTTCTATC90810.11992931816861155No Hit
CCTACAACTTCTTCCGCTCTCGAAAAGACCAATATAATAAAAAGTTATAA90650.11971801224517827No Hit
CACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGAT89390.11805397809814104No Hit
GCTTCAAAGCCTTGACAAGACCTCTTGGGACCCAAGTTCTAATTCTGAAG88610.11702386172140371No Hit
CTTAACTATCTGCTTAGTCGAGGAGAACCAGGATTCTGTTCGTTGCTCAG88300.1166144564947517No Hit
CTCTTCGCGGTTCTGCAGATCATGAATCGGACCAGGAAAACCATACTTGA88180.11645597705217672No Hit
GTTCCCCGTGAGTTGCTTTCTCTGAAAACCTTATTATACATTAGAGTTAT88130.11638994395110382No Hit
GGTGGGTTCTCGGACCTGCTTTGGTCAGACCCTGACAATGTAGAGGCTTG87350.11535982757436647No Hit
GCTCTTTTACATTCGAGCTTGATCTTTTTGTAATCATGCAACGTTCTCAT85830.11335242130175013No Hit
GGATACTTCTCAATCAACTTTACAAAGTGATATGAACATTCCCCGCATGG84250.11126577530784633No Hit
GTGCCGGCTGCAATAATCTAGTCAATTTAGAATCACGATATGGAATGTGG84230.11123936206741715No Hit
CCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGC81500.1076339547488365No Hit
GTATTCAGCCTCACGCGAACATTCAACCGCCTACTGGCATTTTACCTTTA79980.10562654847622016No Hit
GAAATATTATAAAAAACGTTCAAAAATATTTCGAATTTGGGTAGCTTCCA79660.10520393662935357No Hit
CCAAGAGCTAAGTTACCTTCTGGGGTGATGGACCAACCAGCAGCATAGAT79350.10479453140270155No Hit
GCTTTCCATCTGAGGAAGATCGTTGACTATACAATCACCTCTAAACTCGG79340.10478132478248696No Hit
GTCCCTACGGCCTTGTCTAACACCATCCAGCATGCAATACAGTGACATAT78190.10326256345781014No Hit
GCTTCGAAAGGACGTTCCGTTGCTGTTTTGTCTGTATTTAGGAACTAAGA78100.1031437038758789No Hit
CCCCTTATTTCAACGTTATGGATCCCAGATAAAATACATTACTCCAAGAA77520.1023777199034332No Hit
GAACCTATAATTGCTTTTTGTTGAGGACTTATAGTGCTAGAAATGGCTTC77450.10228527356193112No Hit
GTCCACACGGCATTACCGTTTCACTAATTTATTGCCATCTTCCTCCACAG75820.1001325944669544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA257100.065.5889556
TCGTCTC256950.065.5736255
GTACAGC258350.065.5664926
CCGATTG257700.065.2236747
GCCGATT257300.065.1618246
TGCCGAT258400.065.0570945
CTCCATG263000.064.3064232
GGACGAT97950.063.9348728
TTGCCGA264650.063.82108344
GCTCCAT264900.063.81875231
CCGGGAC54750.063.80130819
TTATATG266750.063.6183266
TCCGGGA54900.063.49947718
TGTACAG267250.063.42269525
GGAGCGT29250.063.4159851
GGTTTAA272400.063.341531
TTCGTCT267450.063.03848354
CGGGACT55550.063.0109920
CTTGGAC99700.062.8134825
GTCATGG99850.062.6386614
Produced by FastQC (version 0.11.5)