FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n02_1657_2_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n02_1657_2_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11068467
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAACCGTTACCCTCCAATTACCCATATCCAACTCCACTACCACTTAC1877141.6959349474502659No Hit
GGTTTAAATTTTCAACTAATGCATTGTACAGCTCCATGTTTATTTGCCGA1604401.4495232266582174No Hit
GTACCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTA1300831.1752576034242141No Hit
CCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGC1023880.9250422845367836No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTTGG1006690.9095116785368741No Hit
GCTTTGTACATCTGTCATGGCATTCTTGGACGATTCTGTATACTTCGGAA953200.861185202973456No Hit
GTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAATAATAC858260.775410000318924No Hit
AGAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAA818610.7395875146937693No Hit
ATATTACAACGTCTCCAATGATATCAGCGGTTTTCAGAACAGAATGGGGC776580.7016147764636241No Hit
GCCATACGTCCGTAGTATGGACCCTGCAGGAATGGTCTACATTTTCTATC737980.6667409316936121No Hit
CGTTTGTTTTTAATTATATACAGGTTGGTTAGGTGACGTTGGAGAAAAGA675360.610165798027857No Hit
CCCATATGCTGATCTTTTCCGGGACTTCTAATGACTAATGTTTTGTTGTA675110.6099399311575848No Hit
GATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAAC631760.5707746158524031No Hit
GCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAT504140.4554740959159024No Hit
GGAGCGTTAACACACCATCCATGTGGCCCTTGAATTCCTGAATCCTGCAG464710.419850373136587No Hit
GTTGAAAAAATCGAGGTGGAATTTGAGAACGACAATTACTTAGCACTACA413290.37339407525902185No Hit
CCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGTT401980.36317585804791214No Hit
CACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCT381990.34511554310095516No Hit
GAATGGGGCTGTTCTTCTTTATACTAACGTACTTCGGTTTTGTTACATTC379450.34282073569899063No Hit
CTCAACCATCCACTCCCAACCACCATCCATCTCTCTACTTACCACTAACC367110.3316719469823599No Hit
ACATTTATTTTTTGAAAGAAAGGGGAAGATAGTTATATTAACACCAGAAT362710.32769669006557095No Hit
GATATTGGAATTGGAAATGGCAATTCACTTCCATTTATTTCAGAAATAGT344630.3113619980074928No Hit
GAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAAG321300.2902841016737006No Hit
CTTTTATGACCGTACTTTTAGAGCATCCACTGGGGCCAGACAGTAGTAGG321070.2900763041530503No Hit
CTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGA305870.2763435984405067No Hit
GTTCCTATGATGCCCCAGGCTCAAACTTCAGCAAGTATTCAGCCTCACGC298950.27009160347137506No Hit
GTTTTATTTCCGTGCATATGCATCCGACTTGCAATTAGTCGAAAGATTTC286750.2590693002020967No Hit
CTTTAAACATGGACCTACGCTTAGCCTGCGCTTAGCCTACAACTTCTTCC285790.2582019714202518No Hit
GGATTTATGGATCAAACAACTGTCTCGGACAAGGCAATTATTTTCAAATA284100.2566751113772124No Hit
AACCCGTACTTCCAACTGCAGGACGACCCAACCATCAAAGACACACCAGA255640.23096242686543675No Hit
CCTCAGTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAA251770.22746600771362466No Hit
CTAACAAATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATT246100.22234334709585346No Hit
CCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTACCC241580.21825967408133393No Hit
GGAACTGTCTGTTGGCAACGATAGAGCCAATTCTACCCTTCCTATCGGTT241200.21791635643852036No Hit
GACTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAAT227780.20579182284231412No Hit
GGATAATCAGCTCTGTCGTGACTGATAAATGCTATATCCGGCATATGCAG222810.2013015894613048No Hit
CACACCACACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAA222050.20061495417567762No Hit
GAACCTATAATTGCTTTTTGTTGAGGACTTATAGTGCTAGAAATGGCTTC220480.19919651023036886No Hit
GCTTTTGTATTAGAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATA219380.1982026960011716No Hit
GAACTACCACTTTGTTCATGTGTACAATGTTCATTATCTCCATAAGCAAA208900.18873435679936526No Hit
AGTCCTTCCGGGCTATTTGACCGGACCAACTCTATAATCAGCCTTGATCC202110.18259981260277505No Hit
ATTTCCATGGTGCACAGTATCTTAACTATCTGCTTAGTCGAGGAGAACCA201610.1821480788622309No Hit
GTTCTGGTATGACGCGTACTTGCTATATGATGCAACTGTCGTTCTCAACG199520.18025983182675612No Hit
ACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCG198340.17919374019907183No Hit
CGTCTAAAGACATGGGGTCAATGCCTTTTTGGTTAATGATAACAAAACCC196700.17771205353008687No Hit
CCTTTATTTAGCATGGCCATTGAATGTAACAATTATATATATCGCAAGCA195280.17642912970694133No Hit
CCAATAAACTTGCAGACCAAGAGGTTGTTGACCTCATCACCTCTACGGTG194930.17611291608856042No Hit
GGTTTTGAAATCCATCTGGGATGGCCGCCAGTGAGATTATCTTATTAGGT193540.17485709628984755No Hit
GAACCATTAACACTAAAACAGAATACAGAATATCCTTGTTCAAGGGCTTT193490.17481192291579314No Hit
GCATTATCAAGCATATCTACTGGAAACCAGAGGAAATTTGAGGTAAAACA187060.16900262701239477No Hit
GGGTACCCGTTTTATCGAAGACAACATGCGAAGTGTTGTGAGCAACTTCT186370.16837923445044378No Hit
GTTAAACTCCTTTACAATATCTGGACAGACGTAACAGTATTCTTGCTTAA186050.1680901248564955No Hit
CACACCACACACCACACACTACCCCTAACACTACCCTATTCTAACCCTGA184860.16701499855400032No Hit
GTGATATATCTACGGTAGGAGACGGTACTGAAGACTGAGGAGGTGATGGT184060.1662922245691296No Hit
GCATGGCCATTGAATGTAACAATTATATATATCGCAAGCACAAAAAATCA179400.16208206610725767No Hit
CCCTGCAGGAATGGTCTACATTTTCTATCTACTAGTAAAGCTTTATTCAC174060.15725754975824566No Hit
GACCAACTCTATAATCAGCCTTGATCCGCTGAGTGAGACGCTAACATCAA171640.15507115845401176No Hit
GTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAAGAATA168790.15249627613290984No Hit
CTCTTCGCGGTTCTGCAGATCATGAATCGGACCAGGAAAACCATACTTGA168120.1518909529205806No Hit
CCCTGTAGTGTGGTAGCCTATTTAGTTCATTCTCAGTCAACTGTAATATC167220.15107783218760104No Hit
GTTCCCCGTGAGTTGCTTTCTCCGAAAACCTTATTATACATTAGAGTTAT164760.1488553021841236No Hit
ACCTTCTTTTCCCAGACGTCTAGGACCTCTTGTTCCTTTTGTATACCATG163750.14794280002822432No Hit
CTTAACTATCTGCTTAGTCGAGGAGAACCAGGATTCTGTTCGTTGCTCAG162490.14680443100205295No Hit
GCTCTTTTACATTCGAGCTTGATCTTTTTGTAATCATGCAACGTTCTCAT161840.14621717713934548No Hit
CTCTTTTGTATAGGGCGATGAATTCTGCCTGGTTGACAGTGCCAAACCTG161590.1459913102690734No Hit
CCTTTTTCTTTGAGATAGGATGCTATTGCCATGGAAACTGGGTGTTTTAT161540.14594613689501898No Hit
GCTTTCCATCTGAGGAAGATCGTTGACTATACAATCACCTCTAAACTCGG158190.14291952083337287No Hit
AAATCAAGGAGAGAGAACTACCACTTTGTTCATGTGTACAATGTTCATTA154870.1399200087961594No Hit
GGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTA154820.139874835422105No Hit
CCTACAACTTCTTCCGCTCTCGAAAAGACCAATATAATAAAAAGTTATAA152950.13818535123246967No Hit
CCTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTATT149430.13500514569903854No Hit
GAAATTTGTGTTTACAAAGGCATTTAAAGAAGAATATGGCCTATGTGAGG147500.13326145346053794No Hit
CCGTAGGAAGAACATTTCTTGATCACAGACTCAATTGCATTGTAACCTCT141710.12803037674503615No Hit
GGATCAGATAAGTCTATCGGTTTGCAAATTTTACCTATTAGCGGTATCGG138610.12522962755366213No Hit
GGGTGTTTTATTCCTTCAGTTAATCCAAGCAACAAAGATTGAGAATTATG137540.12426291734889755No Hit
GGGTGGGTTCTCGGACCTGCTTTGGTCAGACCCTGACAATGTAGAGGCTT131880.11914929140593725No Hit
CACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGAT131110.11845362144549919No Hit
ATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAACG129810.11727911372008427No Hit
CAAGAGTATCCTACTGCACATGATAACTTTAACTTTGGGATCTAATAAGT128420.11602329392137141No Hit
GCTTTTGAATCGAGGGGTGGATCCAAAACCTGGGGTAGCCAGACTTTAAA127100.11483071684633474No Hit
GTGCCGGCTGCAATAATCTAGTCAATTTAGAATCACGATATGGAATGTGG126280.11408987351184226No Hit
GTCCGACTAAACCGGACAAGAGTATCCTACTGCACATGATAACTTTAACT125500.11338516887659329No Hit
GCTTCGAAAGGACGTTCCGTTGCTGTTTTGTCTGTATTTAGGAACTAAGA123940.11197575960609542No Hit
CCATCCCGGTTGATCCTGAAAAGGTTGTTGCGATTGTGGAGTCCACCATG123880.1119215515572301No Hit
ATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATTTTTTCAC122070.1102862754164601No Hit
GGATACTTCTCAATCAACTTTACAAAGTGATATGAACATTCCCCGCATGG121140.1094460506590479No Hit
ACCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGT120680.10903045561774724No Hit
CAGTAATCACGCACTCCAAAATCTCCAAACAAATAAAATCGAAGCCTCAA120580.10894010886963841No Hit
GTCCCTACGGCCTTGTCTAACACCATCCAGCATGCAATACAGTGACATAT120260.10865099927569012No Hit
GTACTGTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAAT116610.10535334296971749No Hit
GAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATAGGATGCTATTGC115580.10442277146419643No Hit
CGTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGC114940.10384455227629988No Hit
GTTCCCCGTGAGTTGCTTTCTCTGAAAACCTTATTATACATTAGAGTTAT114010.10300432751888768No Hit
GGTTTTTGAATAATTTCGCCAACACTAGATCGTTTGGTGATGTCGCTGGG113700.10272425259975025No Hit
GTATTCAGCCTCACGCGAACATTCAACCGCCTACTGGCATTTTACCTTTA110700.10001385015648509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGAT108600.064.07851428
GCCGATT181900.063.78835346
TCGTCTC182250.063.4709355
GTACAGC184350.063.44027726
CCGGGAC77750.063.4312319
CCGATTG183250.063.4139347
CGTCTCA183400.063.16693556
TCCGGGA78100.063.14696518
TGCCGAT185250.062.76481645
CGGGACT79250.062.40899720
CTCCATG186950.061.89396732
GTCATGG113450.061.51593814
GGAGCGT59000.061.2803461
GCTCCAT189300.061.2365431
TTGGACG114500.060.80804426
TTGCCGA194200.060.55242544
CTCTCGT81100.060.5045856
GAGCGTT59950.060.4794962
TTCGTCT192300.060.2812154
CTTGGAC115800.060.06466725
Produced by FastQC (version 0.11.5)