FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n02_1657_1_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n02_1657_1_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8866369
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTAAATTTTCAACTAATGCATTGTACAGCTCCATGTTTATTTGCCGA1719951.939858356898974No Hit
CCATAACCGTTACCCTCCAATTACCCATATCCAACTCCACTACCACTTAC1513101.7065610510909257No Hit
GTACCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTA1116421.259162572638247No Hit
GTTGTTCTACCCTCCATATTGAAACGTTAACAAATGATCGTAAATAATAC742690.8376484218060403No Hit
GCCATACGTCCGTAGTATGGACCCTGCAGGAATGGTCTACATTTTCTATC732750.8264375191242324No Hit
GTATTGTAAAAGTGCCAGTATTAATTTAGCAGTGGAGATTTTTTTTTTGG721670.8139408589919955No Hit
GCTTTGTACATCTGTCATGGCATTCTTGGACGATTCTGTATACTTCGGAA689970.7781877790107765No Hit
CCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGC626880.7070312548462623No Hit
AGAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAA528380.5959372996995727No Hit
ATATTACAACGTCTCCAATGATATCAGCGGTTTTCAGAACAGAATGGGGC442370.4989302836369657No Hit
CTCAACCATCCACTCCCAACCACCATCCATCTCTCTACTTACCACTAACC437430.49335866801844136No Hit
CGTTTGTTTTTAATTATATACAGGTTGGTTAGGTGACGTTGGAGAAAAGA433010.48837353825449853No Hit
CCCATATGCTGATCTTTTCCGGGACTTCTAATGACTAATGTTTTGTTGTA419850.47353093470393576No Hit
CCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGTT388450.43811621194651384No Hit
GCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGAT329800.37196737469419555No Hit
GGAGCGTTAACACACCATCCATGTGGCCCTTGAATTCCTGAATCCTGCAG328260.37023047427870415No Hit
GATATCAGCGGTTTTCAGAACAGAATGGGGCTGTTCTTCTTTATACTAAC319960.3608692577536532No Hit
CACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGATGAACCT316990.35751952123806263No Hit
GATATTGGAATTGGAAATGGCAATTCACTTCCATTTATTTCAGAAATAGT296850.3348044729471557No Hit
ACATTTATTTTTTGAAAGAAAGGGGAAGATAGTTATATTAACACCAGAAT280300.31613843276768655No Hit
GACTTTAAGTTTAATTTCCAGTTTTTAGACAGCCTTAATTGTTCAACAAT274360.3094389597365054No Hit
GTTGAAAAAATCGAGGTGGAATTTGAGAACGACAATTACTTAGCACTACA252630.2849306181594743No Hit
CGTCTAAAGACATGGGGTCAATGCCTTTTTGGTTAATGATAACAAAACCC246920.27849055233320424No Hit
GTTCCTATGATGCCCCAGGCTCAAACTTCAGCAAGTATTCAGCCTCACGC231770.26140351253145455No Hit
GGATTTATGGATCAAACAACTGTCTCGGACAAGGCAATTATTTTCAAATA219260.2472940162991186No Hit
CTTTTATGACCGTACTTTTAGAGCATCCACTGGGGCCAGACAGTAGTAGG214250.2416434506617083No Hit
CTTTAAACATGGACCTACGCTTAGCCTGCGCTTAGCCTACAACTTCTTCC211190.23819220697897867No Hit
GAATGGGGCTGTTCTTCTTTATACTAACGTACTTCGGTTTTGTTACATTC209860.23669215662014517No Hit
GAATTCAAATCCTTCAATAATTTTTGATGATTTTTTTTTAGAGTATCAAG208820.23551918491098214No Hit
CCCTATTCTAACCCTGATGAACCTGTCTCCAAACCTACCCTCCATTACCC202020.2278497545049163No Hit
GTTTTATTTCCGTGCATATGCATCCGACTTGCAATTAGTCGAAAGATTTC196970.2221540745709997No Hit
CTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGA195350.22032694556249577No Hit
GGAACTGTCTGTTGGCAACGATAGAGCCAATTCTACCCTTCCTATCGGTT188960.21311993669561913No Hit
CTCTTCGCGGTTCTGCAGATCATGAATCGGACCAGGAAAACCATACTTGA186610.21046947177587577No Hit
GGATAATCAGCTCTGTCGTGACTGATAAATGCTATATCCGGCATATGCAG180480.2035557058362899No Hit
GAACTACCACTTTGTTCATGTGTACAATGTTCATTATCTCCATAAGCAAA179860.20285643424044272No Hit
AGTCCTTCCGGGCTATTTGACCGGACCAACTCTATAATCAGCCTTGATCC175170.19756678297508257No Hit
GAACCATTAACACTAAAACAGAATACAGAATATCCTTGTTCAAGGGCTTT172890.1949952680742252No Hit
CACACCACACACCACACACTACCCCTAACACTACCCTATTCTAACCCTGA171550.19348393914126516No Hit
ATTTCCATGGTGCACAGTATCTTAACTATCTGCTTAGTCGAGGAGAACCA167360.18875821658223338No Hit
AACCCGTACTTCCAACTGCAGGACGACCCAACCATCAAAGACACACCAGA167250.18863415226684113No Hit
CCTTTATTTAGCATGGCCATTGAATGTAACAATTATATATATCGCAAGCA166540.18783337350385484No Hit
CCCTGCAGGAATGGTCTACATTTTCTATCTACTAGTAAAGCTTTATTCAC165560.18672807323945123No Hit
GCTTTTGTATTAGAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATA164940.18602880164360405No Hit
GCATGGCCATTGAATGTAACAATTATATATATCGCAAGCACAAAAAATCA164740.1858032301610727No Hit
GAACCTATAATTGCTTTTTGTTGAGGACTTATAGTGCTAGAAATGGCTTC152820.17235916980220425No Hit
CCTCAGTGTCCTTATCATTGAAATTTGTGTTTACAAAGGCATTTAAAGAA151020.1703290264594221No Hit
CACACCACACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAA147770.1666634898682877No Hit
GTGATATATCTACGGTAGGAGACGGTACTGAAGACTGAGGAGGTGATGGT141890.160031688281866No Hit
CTAACAAATTACTTGATGAGTGACCAATAACCAATTGCACCATTGATATT140570.1585429164971591No Hit
GCTCTTTTACATTCGAGCTTGATCTTTTTGTAATCATGCAACGTTCTCAT139660.15751656625164145No Hit
GTTCTGGTATGACGCGTACTTGCTATATGATGCAACTGTCGTTCTCAACG138840.15659172317326292No Hit
GCATTATCAAGCATATCTACTGGAAACCAGAGGAAATTTGAGGTAAAACA136600.1540653225689118No Hit
GGGTGGGTTCTCGGACCTGCTTTGGTCAGACCCTGACAATGTAGAGGCTT132280.14919297854623464No Hit
AAATCAAGGAGAGAGAACTACCACTTTGTTCATGTGTACAATGTTCATTA131670.14850498552451402No Hit
CAGTAATCACGCACTCCAAAATCTCCAAACAAATAAAATCGAAGCCTCAA130340.14700493516568056No Hit
GACCAACTCTATAATCAGCCTTGATCCGCTGAGTGAGACGCTAACATCAA129300.14583196345651753No Hit
CCCTGTAGTGTGGTAGCCTATTTAGTTCATTCTCAGTCAACTGTAATATC128230.14462515602497483No Hit
CTCTTTTGTATAGGGCGATGAATTCTGCCTGGTTGACAGTGCCAAACCTG127180.14344090574168522No Hit
GGGTACCCGTTTTATCGAAGACAACATGCGAAGTGTTGTGAGCAACTTCT127110.14336195572279925No Hit
CCTACAACTTCTTCCGCTCTCGAAAAGACCAATATAATAAAAAGTTATAA122440.13809486160569226No Hit
CTTAACTATCTGCTTAGTCGAGGAGAACCAGGATTCTGTTCGTTGCTCAG118970.13418119638377335No Hit
CCTTTTTCTTTGAGATAGGATGCTATTGCCATGGAAACTGGGTGTTTTAT118420.1335608748068121No Hit
CACCCACACACCCTAACACAATCCTAACAGTACCCTATTCTAACCCTGAT115710.13050438121851232No Hit
GGTTTTGAAATCCATCTGGGATGGCCGCCAGTGAGATTATCTTATTAGGT114030.128609580765249No Hit
GTTAAACTCCTTTACAATATCTGGACAGACGTAACAGTATTCTTGCTTAA113880.12844040215335048No Hit
CCAATAAACTTGCAGACCAAGAGGTTGTTGACCTCATCACCTCTACGGTG112120.1264553731070746No Hit
ACCTTCTTTTCCCAGACGTCTAGGACCTCTTGTTCCTTTTGTATACCATG111890.12619596590216356No Hit
GAAATTTGTGTTTACAAAGGCATTTAAAGAAGAATATGGCCTATGTGAGG108050.12186499343756164No Hit
ACCCGGAAGGATCGACGTTGAAAGAGTGAGATTGAATATTACTATTAGGT107510.121255950434727No Hit
GTGCCGGCTGCAATAATCTAGTCAATTTAGAATCACGATATGGAATGTGG105550.11904534990591978No Hit
CCTAAACTATTATTGGGTAATTATTTACTGACGATCCTATTGAGTTTATT105020.1184475854772117No Hit
GCTTTTGAATCGAGGGGTGGATCCAAAACCTGGGGTAGCCAGACTTTAAA103930.11721822089741585No Hit
GTCCGACTAAACCGGACAAGAGTATCCTACTGCACATGATAACTTTAACT99770.11252633406076376No Hit
GCTTCATCTTGGTTTATTGTAGCATGAATCATATTTGCCTTTTTTTCCTG99360.1120639125215745No Hit
CAAGAGTATCCTACTGCACATGATAACTTTAACTTTGGGATCTAATAAGT98900.11154509811175241No Hit
GAAACATTTTGAGCAGAAACACCTTTTTCTTTGAGATAGGATGCTATTGC98770.11139847664810702No Hit
ACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCG97710.11020294779069087No Hit
GCTTCAAAGCCTTGACAAGACCTCTTGGGACCCAAGTTCTAATTCTGAAG94160.10619905397575942No Hit
GGTGGGTTCTCGGACCTGCTTTGGTCAGACCCTGACAATGTAGAGGCTTG93550.1055110609540388No Hit
GGGTGTTTTATTCCTTCAGTTAATCCAAGCAACAAAGATTGAGAATTATG92820.10468772504279937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGAT77850.064.883628
GTACAGC194000.064.7231126
CCGATTG194000.064.4477247
GCCGATT194250.064.2206246
CGTCTCA195950.063.8549256
TCGTCTC196100.063.79110755
TGCCGAT196250.063.45591445
CTCCATG197800.063.2240832
CGGGACT50400.062.64495520
GTCATGG82200.061.90828714
TTGGACG81800.061.7508226
TTCGTCT202750.061.75060354
TTGCCGA202450.061.7328144
TCCGGGA51200.061.66195718
TGTACAG203550.061.6531325
GCTCCAT203000.061.64042331
CCGGGAC51500.061.09921319
CGTCCGT87850.060.9909177
CTTGGAC83500.060.6623325
TTATATG207150.060.65180266
Produced by FastQC (version 0.11.5)