FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42806842
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTTG452400.0103.3573645
GGGATCC198650.0103.01179564
TCCCCCG197400.0102.9384268
GATCCCC199350.0102.4354366
TTCTCTG453500.0102.156358
ATCCCCC199350.0102.03097567
CCCCCGG199450.0101.6879269
CCTCGAG77900.0101.1790763
CCCCGGG200450.0100.8510370
TAGGGTA262050.0100.5860553
AGGCTCC89100.0100.49602554
GTTCGGA72850.0100.0302844
CACTTGT466550.099.9703646
ACTTGTT471650.099.8030347
CTGGGAT206100.099.57130462
CCCATAG89050.099.5125451
TCCAGAG99100.099.49348468
CCAGAGC98550.099.21194569
GGGTAAG263450.099.1007955
TCCTCGA79350.099.0466762
Produced by FastQC (version 0.11.5)