FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n01_1751_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n01_1751_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11514138
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGG750.070.035341
CCCCTCG207.91185E-570.035331
TCATAAG11313400.069.78268
ATCATAA2395550.069.697577
TAAGTAG11317050.069.6577311
AAGTAGC11371800.069.53133412
CTCATAA1873250.069.505287
CGTCATA548750.069.469386
GTAGCAA11282650.069.4559914
AGCAAGT11159950.069.4453216
TAGCAAG11224150.069.4107515
CAAGTGG10955100.069.3928118
GCAAGTG11057250.069.3889117
GGTCATA783900.069.337316
GCGTCGC421250.069.3338624
TCTCATA690300.069.286446
CCTCGAG214850.069.2654863
AAGTGGC10866850.069.2564819
GTCACCC79400.069.2491844
CTTCATA652700.069.244676
Produced by FastQC (version 0.11.5)