FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n01_1747_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n01_1747_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8124969
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG301.0217627E-770.032771
TAGGCCG252.8346585E-670.032761
TCATAAG8000450.069.780858
TAAGTAG8001300.069.6763411
ATCATAA1681700.069.638677
AAGTAGC8038450.069.55765512
CTCATAA1350750.069.5400857
CGTCATA397800.069.525936
GTAGCAA7986700.069.5136114
AGCAAGT7912100.069.50548616
GTGGCAC295050.069.500521
TAGCAAG7947850.069.4724815
CAAGTGG7782850.069.4581818
GCAAGTG7851350.069.44729617
GGTCATA547300.069.4470756
CCGGGAC100800.069.4431737
GGCACCG203200.069.4130923
CCCGGAG100800.069.4084543
ACCCGGA100850.069.3740342
GCTCATA344250.069.36936
Produced by FastQC (version 0.11.5)