FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n01_1744_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n01_1744_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10428410
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAG207.908325E-570.041571
CCGGGCA301.0208896E-770.041571
ACGGGCC401.3460522E-1070.041571
AGGTCGG455.456968E-1270.041571
TCATAAG10259950.069.7574168
TAAGTAG10265700.069.6123211
CTCATAA1725600.069.6007167
ATCATAA2152750.069.5885547
TCTCATA643900.069.5138556
CGTCATA508300.069.511286
AAGTAGC10316150.069.4961412
GTAGCAA10255750.069.4542814
AGCAAGT10139300.069.450116
TAGCAAG10203450.069.4130815
CAAGTGG9936300.069.40755518
GCAAGTG10023100.069.3718217
CGCATAA909750.069.3557825
CCGCGCA21650.069.3516267
GCCACTT903100.069.3161844
CCCGGAG147750.069.311343
Produced by FastQC (version 0.11.5)