FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n01_1740_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n01_1740_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7954179
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG207.916046E-570.027571
CTCGCAC252.8359027E-670.027571
ACGTCTG800.070.027571
GCCCGCG650.070.027571
CCTCGGC150.00222191870.0275651
ACGTCCG353.7052814E-970.0275651
GTCGGCC353.7052814E-970.0275651
TCATAAG7819550.069.755918
ATCATAA1631550.069.636437
CTCATAA1341150.069.6362157
TAAGTAG7823600.069.63167611
AAGTAGC7863750.069.51427512
AGCAAGT7730500.069.4683416
GTAGCAA7814750.069.4530114
CAAGTGG7597750.069.4314318
TAGCAAG7769750.069.4291215
TCTCATA485400.069.409226
GCAAGTG7666600.069.40913417
GCGCATA1149100.069.4019824
CGCATAA1139450.069.3999925
Produced by FastQC (version 0.11.5)