FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n01_1736_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n01_1736_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8696712
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCC500.070.032791
TACGCGG301.0217627E-770.032781
AGGCCGC301.0217627E-770.032781
TCCGTCG550.070.032781
TTCGCGC207.913167E-570.032781
TCATAAG8552050.069.748118
ATCATAA1813100.069.633737
TAAGTAG8555900.069.6291711
ACGTCGG47650.069.5578631
AAGTAGC8598800.069.5007712
AGCAAGT8432950.069.458216
GGTCATA573200.069.45616
CTCATAA1429800.069.445357
GTAGCAA8534950.069.442114
CAAGTGG8282950.069.4226318
TAGCAAG8482900.069.4151715
GCAAGTG8351200.069.41350617
CAACGGC244150.069.3391443
AAGTGGC8211900.069.2970319
GCGGTGG861950.069.296124
Produced by FastQC (version 0.11.5)