FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n01_1734_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n01_1734_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11449328
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCTA550.070.039851
AGGTCCG600.070.039851
CCCCTAG207.909325E-570.039841
CTCGGAC207.909325E-570.039841
TCATAAG11265550.069.760278
ATCATAA2374350.069.6503757
TAAGTAG11271700.069.64023611
CTCATAA1913650.069.59047
AAGTAGC11331600.069.51240512
CGTCATA550600.069.505816
AGCAAGT11145150.069.4603216
GTAGCAA11260100.069.44591514
GGTCATA765000.069.4424446
TAGCAAG11199800.069.424515
CAAGTGG10980800.069.4226218
GCAAGTG11060500.069.41949517
TCTCATA680650.069.371566
GCGCATA2314000.069.3403224
ATCCCCC1660300.069.3300267
AAGCCAC2286500.069.32474542
Produced by FastQC (version 0.11.5)