FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n01_1728_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n01_1728_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7571960
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGG500.070.033691
ACGTTCG207.9125966E-570.033681
CCTAGGC401.3460522E-1070.033681
TGTCGGG353.7034624E-970.033681
TCATAAG7447600.069.773448
TAAGTAG7452250.069.6453611
CTCATAA1242450.069.60987
ATCATAA1569300.069.5948647
AAGTAGC7489650.069.5340412
GGTCATA514450.069.508416
CGGGACC103650.069.45827538
AGCAAGT7363350.069.4538316
GTAGCAA7444100.069.4512814
CGTCATA371050.069.4458856
GAGAGGC142800.069.4348370
TAGCAAG7401900.069.41080515
CAAGTGG7238900.069.4101318
GCAAGTG7298000.069.40147417
CCCGGAG104100.069.39336443
TCTCATA450550.069.390386
Produced by FastQC (version 0.11.5)