FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n01_1657_2_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n01_1657_2_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11068467
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATAAG10877000.069.758378
CGCTCGG32100.069.6713244
TAAGTAG10885500.069.6296311
ATCATAA2289800.069.602547
CTCATAA1829800.069.581437
AAGTAGC10941450.069.5013212
GTAGCAA10866050.069.44569414
AGCAAGT10751600.069.4401316
CACGGGG84850.069.4209369
TAGCAAG10807450.069.4190615
CAAGTGG10553400.069.4001318
GCAAGTG10633400.069.3947617
GCGTCGC356400.069.3306424
GAGAGGC115000.069.32886570
GGTCATA740450.069.327126
CGTCATA542650.069.322396
AAGTGGC10454150.069.29233619
GGCGTCG358950.069.2768823
TCTCATA680550.069.25656
AGTAGCA10965000.069.2457613
Produced by FastQC (version 0.11.5)