FastQCFastQC Report
Wed 18 Sep 2019
HTFWFAFXY_n01_1657_1_tninsertions.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFWFAFXY_n01_1657_1_tninsertions.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8866369
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG700.070.0331341
TCATAAG8677800.069.7612158
ATCATAA1837200.069.644437
TAAGTAG8681800.069.630311
AAGTAGC8722000.069.5184412
CTCATAA1456350.069.506117
CGTCATA430850.069.464696
AGCAAGT8549800.069.4563716
TAGCAAG8596700.069.4410115
GTAGCAA8657900.069.44050614
CAAGTGG8384950.069.4014618
GCAAGTG8457300.069.4007817
CCCGGAG152450.069.3789143
GCTCGCC153050.069.3584732
TCTCATA522650.069.3526846
CTCGCGG73050.069.3280131
GGTCATA581150.069.298286
AAGTGGC8306400.069.2660419
AGTAGCA8739250.069.2562713
CCTCGAG164850.069.25568463
Produced by FastQC (version 0.11.5)