FastQCFastQC Report
Tue 23 Mar 2021
HTFHVBGXG_n02_10X_CD8_v3_1_3_TCR_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFHVBGXG_n02_10X_CD8_v3_1_3_TCR_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26409678
Sequences flagged as poor quality0
Sequence length132
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC19151527.251705227151955No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC8517213.225033641076578No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC6270272.3742319008963304No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC2866391.085355906270421No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT845320.3200796314139082No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC627520.2376098640808873No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTA404530.15317490807725864No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGAAAG400740.1517398281039246No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC396800.15024795077016842No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTGC357710.13544655864414554No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG350270.13262940956720487No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCTG338980.1283544615727613No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG331800.1256357612538858No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG322320.12204616807520334No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA316290.11976291418623126No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGCTCTT291080.11021717114460843No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATAGGGCTGG290610.11003920608195224No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT286750.10857762067375451No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATTTGGTTTT284340.10766507641630466No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC281030.10641174799632167No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATGGGGTTTG274000.10374984503786831No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGCTTCTGGATATCAGGCCAG268510.10167106164641612No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG268320.10159911832321468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)