FastQCFastQC Report
Tue 23 Mar 2021
HTFHVBGXG_n02_10X_CD8_v3_1_3_ORF_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFHVBGXG_n02_10X_CD8_v3_1_3_ORF_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19484940
Sequences flagged as poor quality0
Sequence length132
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTG3183071.6336052356332633No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT2745071.4088162447510746No Hit
AGGAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAG2094881.0751277653408222No Hit
GGAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAGC1973011.0125820248869126No Hit
GTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAAG1433680.7357887681460656No Hit
CCGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGA1324110.6795555952443272No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATG1182330.6067917068258871No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGGT1160270.595470142581912No Hit
GTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCAC896320.4600065486473143No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT877460.450327278400652No Hit
CCGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACC815050.41829741328431086No Hit
GAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAGCA804580.41292403261185306No Hit
GTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGACTT531080.272559217529025No Hit
CCGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGAC505230.25929256133198253No Hit
CGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAA458420.23526887945254127No Hit
CGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG447110.22946439660578888No Hit
AAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAGCAA288250.14793476397669175No Hit
CGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCA266230.13663372840768306No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259440.13314898583213497No Hit
ACCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC250780.1287045277019072No Hit
GTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGCAG221470.11366214112027032No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT211820.10870959828462393Clontech SMART CDS Primer II A (100% over 26bp)
TGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGT207300.10638985801341959No Hit
CCGTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGC205200.10531210257768307No Hit
GTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTCCTT204620.10501443679067013No Hit
GTGGGCTTGTACTTCTTGCCCTTGTAGAATTTCCTGAGGTTTTCTTTCTG201280.10330029243097491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)