FastQCFastQC Report
Tue 23 Mar 2021
HTFHVBGXG_n02_10X_CD8_v3_1_3_ORF_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFHVBGXG_n02_10X_CD8_v3_1_3_ORF_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17108441
Sequences flagged as poor quality0
Sequence length132
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTG2787981.6295932516586404No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT2408941.4080417964442231No Hit
AGGAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAG1856831.08532975038462No Hit
GGAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAGC1726281.009022388422183No Hit
GTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAAG1258040.7353329271790456No Hit
CCGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGA1163210.6799041478998583No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATG1031350.6028310820372237No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGGT1022550.5976874222496369No Hit
GTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCAC784470.45852804472365427No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT771080.45070149875140586No Hit
CCGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACC716890.41902707558216434No Hit
GAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAGCA706350.4128663739729412No Hit
GTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGACTT463040.2706500259141087No Hit
CCGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGAC444130.25959700243873773No Hit
CGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAA399490.23350461915261594No Hit
CGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG393710.23012616988304196No Hit
AAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAGCAA253270.14803803572751018No Hit
CGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCA229840.13434304154306054No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221460.12944487460897228No Hit
ACCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC215940.1262183971058497No Hit
GTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGCAG196460.1148322047578736No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT190040.11107967114011147Clontech SMART CDS Primer II A (100% over 26bp)
CCGTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGC180650.10559115234403883No Hit
GTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTCCTT178630.10441044862007005No Hit
TGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGT178150.10412988535892898No Hit
GTGGGCTTGTACTTCTTGCCCTTGTAGAATTTCCTGAGGTTTTCTTTCTG173540.10143530903838638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)