FastQCFastQC Report
Tue 23 Mar 2021
HTFHVBGXG_n02_10X_CD8_v3_1_1_ORF_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFHVBGXG_n02_10X_CD8_v3_1_1_ORF_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18216765
Sequences flagged as poor quality0
Sequence length132
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTG3863932.1210846162861516No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT3151271.7298735532900602No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGGT1288010.7070465035916091No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATG1229400.6748728437787939No Hit
GTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAAG1127570.6189737859603502No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT1001680.5498671141665383No Hit
CCGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGA983420.5398433805343594No Hit
AGGAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAG842280.4623652992175065No Hit
GGAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAGC824410.4525556540911627No Hit
GTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGACTT550890.30240824866544636No Hit
GTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCAC521140.28607713828443193No Hit
CCGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGAC503490.27638826103317465No Hit
CGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG473270.25979914655538455No Hit
CCGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACC449870.24695383620527575No Hit
CGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAA340140.1867181137814535No Hit
GAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAGCA332810.18269434776152627No Hit
TGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGT263010.14437799466590254No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG244500.1342170248120344No Hit
GGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGTTT238840.131109996752991No Hit
GTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGCAG231250.12694350506250698No Hit
GTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTCCTT230820.12670745876120157No Hit
CCGTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGC200210.10990425577757633No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT193850.10641296629780314Clontech SMART CDS Primer II A (100% over 26bp)
CCGTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTCC190010.10430501793265709No Hit
ACCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC189800.10418973950643816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)