FastQCFastQC Report
Tue 23 Mar 2021
HTFHVBGXG_n02_10X_CD8_v3_1_1_ORF_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFHVBGXG_n02_10X_CD8_v3_1_1_ORF_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20329018
Sequences flagged as poor quality0
Sequence length132
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTG4307882.1190792393415165No Hit
CCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCT3507711.725469474226448No Hit
GTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGGT1435200.7059858966134026No Hit
CCGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATG1371430.6746169441140737No Hit
GTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAAG1262860.6212105277293768No Hit
CGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTT1111580.5467947345021781No Hit
CCGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGA1094520.5384027895494017No Hit
AGGAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAG940280.46253094960120555No Hit
GGAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAGC920810.4529535071492386No Hit
GTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGACTT613120.3015984343168962No Hit
GTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACCAC580490.2855474868486023No Hit
CCGTGGGCTTGTACTCGGATGATGATCTTGTGCTCCAAGGGGTGGGGGAC557850.2744106970636752No Hit
CGTGGGCTTGTACTCCACGGTTTCCACGTTGAAGCCTATGGTGGGAATGG520760.2561658413603648No Hit
CCGTGGGCTTGTACTCGTCGATGGCGTGGAAGTCGCCCGTGTCGGCCACC498560.24524549095288323No Hit
CGTGGGCTTGTACTCTCCGGGAAAGGCGTTGGTGGTGTAGCTGATGAGAA381710.18776607901080122No Hit
GAAGAGTTCTTGCAGCTCGCGTGTAGAACCAGTTCTCATCGTAGGGAGCA374770.1843522397392732No Hit
TGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGT289480.14239743405215147No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268430.13204277747208448No Hit
GGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGCTTGTTT266040.13086711812641416No Hit
GTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTCCTT259200.12750246962248743No Hit
GTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGCAG258760.12728603024504184No Hit
CCGTGGGCTTGTACTCTTTGGGGAAAGCGTTAGTTGTGTAGCAGATGAGC227540.11192867259992587No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT217680.10707846291444081Clontech SMART CDS Primer II A (100% over 26bp)
ACCGTGGGCTTGTACTCGGTCGGTCCAGGATTCTCTTCGACATCTCCGGC212400.10448119038509386No Hit
CCGTGGGCTTGTACTCTGTCACTGTCTTTATGTTTCCGTTGATGACCTCC211310.10394501101824005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)