HTFH5AFXY_n02_undetermined.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HTFH5AFXY_n02_undetermined.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30326402
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1633474	5.386309922291474	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	987340	3.255710980814671	Illumina Single End PCR Primer 1 (100% over 50bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	42902	0.14146749093413719	Illumina Single End PCR Primer 1 (98% over 50bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	42142	0.13896142377852805	Illumina Single End PCR Primer 1 (98% over 50bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	34566	0.11397989118524512	Illumina Single End PCR Primer 1 (98% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GCACACG	13445	58.949806	18
GTCCCCG	26500	58.912903	45
TGGTCCC	30730	58.46035	43
GTGGTCC	29695	58.044815	42
GGTCCCC	30335	57.94433	44
AGAGCAC	25750	56.463703	15
CCCCGTA	34675	56.058956	47
TCCCCGT	28670	55.905235	46
GTCCCCA	11965	55.89488	45
TCCCCAT	21380	55.832714	46
CCCCATA	25960	55.65326	47
GTTCTCC	27355	55.232956	42
TTCTCCC	27590	54.96742	43
GCACGCG	5240	54.71663	18
TACCCCG	11170	54.32753	45
TTGTCCC	18035	54.209873	43
GACTAGT	47985	54.100266	12
GACCCCG	9380	54.024376	45
ACCCCGA	15440	53.944157	46
TAACTGA	39295	53.843834	2