FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876014
Sequences flagged as poor quality0
Sequence length76
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5089938.662215576749817Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG571050.9718322658863644No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC185600.3158603774599584Illumina Single End PCR Primer 1 (100% over 49bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC117940.2007142937372171Illumina Single End PCR Primer 1 (98% over 50bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC107400.18277696411206645Illumina Single End PCR Primer 1 (98% over 50bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC94580.1609594531258775Illumina Single End PCR Primer 1 (98% over 50bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC90820.1545605575480249Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC78050.13282813825834996Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC70100.11929855851262439Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC64450.10968319680654266Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC63900.10874718814488869Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC63850.1086620964483747Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG43450.056.3674961
AGATAGA200400.052.9728937
GATCGAG41450.052.9428378
AGAGCAC46300.052.84150315
TGACTGG47750.052.1564562
GAGCACA43350.051.67241716
CAGATAG68300.050.6334346
GATAGAT187400.050.314398
GTGGTCC61550.050.15048242
GTCCCCG58900.050.09506645
CCCCGTA63550.050.06463247
TGGTCCC62800.049.9324643
GGTCCCC62000.049.56323244
TCCCCGT58750.048.97186346
GACCGAC21500.048.83677710
CCCGTAA29600.047.65363348
GACTGGA57400.047.644653
TCGCAAC22650.047.59339510
AAGACCG55900.047.3967178
CGATCGG9700.047.3195841
Produced by FastQC (version 0.11.5)