HTFH5AFXY_n02_ncm4-8.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HTFH5AFXY_n02_ncm4-8.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3863552
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	470714	12.183451911608799	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	37572	0.9724729989398356	No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	15633	0.40462765869334744	Illumina Single End PCR Primer 1 (100% over 49bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	11175	0.28924160979326796	Illumina Single End PCR Primer 1 (98% over 50bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	9775	0.25300552445003976	Illumina Single End PCR Primer 1 (98% over 50bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	8924	0.23097916114497746	Illumina Single End PCR Primer 1 (98% over 50bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	8325	0.21547529320169626	Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	6925	0.17923920785846809	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC	6037	0.15625517658362048	Illumina Single End PCR Primer 1 (98% over 50bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	5847	0.15133742214418236	Illumina Single End PCR Primer 1 (100% over 49bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC	5781	0.14962914954943016	Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	5711	0.14781734528226875	Illumina Single End PCR Primer 1 (100% over 40bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC	5553	0.1437278442221044	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCCCC	5288	0.1368688709249934	Illumina Single End PCR Primer 1 (98% over 50bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	4897	0.12674864994699178	Illumina Single End PCR Primer 1 (98% over 50bp)
AGACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	4303	0.11137419659422211	Illumina Single End PCR Primer 1 (97% over 48bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTGACTG	3475	61.30629	1
GATCGAG	3155	58.01811	8
GGATCGG	1940	57.255047	1
TGACTGG	3820	57.22447	2
GACCGAC	1900	56.183323	10
AGATAGA	16240	56.035053	7
CAGATAG	5445	55.798485	6
CCCGTAA	2695	55.456547	48
CCCCCAG	230	54.832146	2
CCCCATA	1930	54.587463	47
ACCTACG	1080	54.119522	11
GAGCACA	2530	53.949627	16
GATAGAT	14965	53.721172	8
CCTACGT	1060	53.49388	12
GTCCCCG	5595	53.42463	45
TCGCAAC	2005	53.41562	10
TGGTCCC	5930	53.411926	43
GAGACTA	2510	53.26609	10
TCCCCAT	1750	53.20192	46
ACCGACG	2160	52.98528	11

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content