FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6856378
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC6369079.28926322323536Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG573630.8366370698931711No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC259940.3791214545055713Illumina Single End PCR Primer 1 (100% over 49bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC189230.2759912011852322Illumina Single End PCR Primer 1 (98% over 50bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC145550.21228409518845082Illumina Single End PCR Primer 1 (98% over 50bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC137390.2003827676945466Illumina Single End PCR Primer 1 (98% over 50bp)
GGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC136960.19975561440749037Illumina Single End PCR Primer 1 (98% over 50bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC115590.1685875545368123Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC100910.14717683301591597Illumina Single End PCR Primer 1 (100% over 49bp)
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC90170.1315125857996744Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC82310.12004880711069313Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATCTCGGTGGTCGCC78650.11471071169063317Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC78300.11420023808488972Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCCCC70680.10308649843984681Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC69200.10092792433556026Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGG12550.056.1027261
GTGACTG48550.055.917031
GATCGAG44350.055.240268
TGACTGG53450.053.4014172
GGATCGG32550.053.3780021
TCGCAAC33400.053.3358810
GACCGAC28300.052.80676310
AGATAGA226250.052.765597
CAGATAG79800.052.281746
CCCCGTA96950.051.80721347
GTGGTCC88150.051.77036342
GACTAGT57600.051.47366712
TGGTCCC91650.051.20653543
GTCCCCG87700.051.20509745
CCCGTAA45350.051.01645748
GAGACTA48800.050.84807610
AGAGCAC49700.050.7804715
GAGCACA45250.050.74355716
CCCCATA32450.050.69548847
GATAGAT206350.050.6110848
Produced by FastQC (version 0.11.5)