FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9843136
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3095183.1445059785824356Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG807770.8206429333090592No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC126070.1280790999941482Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCG44500.043.5740645
CCCCGTA50400.043.3345747
GGTCCCC47400.042.82670644
AGAGCAC55650.040.69505715
TCCCCGT47700.040.50409746
TGGTCCC51200.040.4653843
GTGGTCC52450.039.3004342
GAGCACA53750.038.94104416
AGATAGA152700.038.392987
GTGACTG38300.038.1480751
GATCGAG31850.036.8119938
GACCGAC18450.036.800610
TGACTGG43150.036.4479262
GACTGGA46500.036.4472663
AAAGGGG716900.036.19780767
AAGGGGG732250.035.5963668
GACTAGT36100.034.80974612
GATCCGA45900.034.617628
CCCGTAA29500.034.40776448
AAGACCG47850.034.304018
Produced by FastQC (version 0.11.5)