FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8191174
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2859993.4915507838070585Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG677670.8273173051872662No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC108320.13223989625907104Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA45750.046.1324747
GTCCCCG41400.044.80804445
GACCGAC16650.044.562310
GGTCCCC44350.043.6424744
GTGACTG35400.042.6644971
TGGTCCC45900.042.54426243
GTGGTCC45300.041.79444542
TCCCCGT44600.041.2007346
CCGACGT18800.041.14755612
TGACTGG40350.040.4565622
AGATAGA135300.039.1386387
GACTAGT37900.038.6052812
GACTGGA45750.037.4305653
AAGACCG44750.037.1489528
ACCCCGA13800.036.77641746
CCCGTAA26000.036.61643248
GATCGAG27550.036.459138
CCCCGAA19900.036.40807747
AGAGCAC40200.036.3956315
ACCGACG21300.036.31292711
Produced by FastQC (version 0.11.5)