HTFH5AFXY_n02_ncm4-3.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HTFH5AFXY_n02_ncm4-3.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7518512
Sequences flagged as poor quality	0
Sequence length	76
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	457134	6.080112660590287	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	72448	0.9635949241020032	No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	13660	0.18168488658394108	Illumina Single End PCR Primer 1 (100% over 49bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	11273	0.14993658319624947	Illumina Single End PCR Primer 1 (98% over 50bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	10680	0.14204938423986024	Illumina Single End PCR Primer 1 (98% over 50bp)
AAATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	7670	0.10201486677151012	Illumina Single End PCR Primer 1 (98% over 50bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CCCCGTA	5225	50.374294	47
GTGACTG	4400	50.170456	1
GTCCCCG	4710	49.86307	45
TCCCCGT	4865	47.98665	46
AGATAGA	19555	47.968307	7
TGGTCCC	5095	47.94171	43
TGACTGG	4760	47.394005	2
GGTCCCC	5125	47.12171	44
GTGGTCC	5120	46.95672	42
GACCGAC	1905	46.6655	10
CCGACGT	2240	45.003666	12
ACCGACG	2285	44.72566	11
CAGATAG	7515	44.666454	6
GACTGGA	5590	44.226665	3
AAGACCG	5140	43.919136	8
GATAGAT	19305	43.637863	8
CCCGTAA	2770	43.340286	48
AGAGCAC	4290	42.8348	15
GATCGAG	4105	42.544613	8
GACCCCG	1045	42.536804	45