FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11605379
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4590023.9550797953259433Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1025940.8840211077983752No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC188380.16232128222611256Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA62200.047.04329347
GTGACTG67350.047.0241431
GTCCCCG56650.045.9679245
TGACTGG74200.044.650422
AGATAGA240550.044.6245047
GATCGAG49250.043.7041448
TCCCCGT60250.043.0468246
TGGTCCC62050.042.80825843
GACTGGA84100.042.2172933
GGTCCCC62900.042.1219444
AGAGCAC69650.041.962415
GTGGTCC62250.041.77120642
GACCGAC25100.041.27318610
GATAGAT239700.040.4304588
GAGCACA65200.039.9942916
CAGATAG94450.039.947646
GACTAGT45850.038.47616212
CCCGTAA37700.038.2505548
CCGACGT29350.038.0437312
ACTGGAG97250.037.937964
Produced by FastQC (version 0.11.5)