FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9010259
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2631972.9210814028764323Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG765640.8497424990779955No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC118010.13097292763726326Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA40800.042.54857647
GTCCCCG37200.042.1498645
GACCGAC16550.041.23686610
GTGACTG39250.040.7951051
AGAGCAC51900.039.9923915
GGTCCCC40900.039.7899944
GAGCACA47800.039.32185416
TCCCCGT41600.038.44890246
TGGTCCC43050.037.7202543
TGACTGG44150.037.685662
AGATAGA138650.037.562447
GTGGTCC42750.037.0842442
GACTGGA49400.035.3715553
GATCGAG29150.034.218068
CCGACGT20600.034.15297312
ACCGACG20300.033.9642211
CCGTGGT42650.033.3171746
CTCCGTG30150.033.31492244
CGATCGG10800.033.0908471
GATAGAT141100.032.9397358
Produced by FastQC (version 0.11.5)