FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6238045
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1705362.7338052226298464Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG580400.9304197068151961No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC76310.12232999281024744Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG28050.041.0996361
CCCCGTA25250.040.6146747
AGAGCAC37750.040.51866515
GTCCCCG23350.039.1225245
TGACTGG31800.039.1057172
GAGCACA36250.038.4286616
GACTGGA36300.036.7524763
GGTCCCC25250.036.59273544
GACCGAC10600.035.65908410
TGGTCCC27350.035.18955243
AGATAGA89400.034.802997
GTGGTCC26850.034.8018142
GGATCGG19100.034.3070341
AAAGGGG397800.034.1706267
TCCCCGT26850.034.15310746
ACTGGAG39900.033.775584
AAGACCG27450.033.4047168
GATCGAG18900.033.331868
AAGGGGG423050.032.79162668
GATAGAT86100.032.5595368
Produced by FastQC (version 0.11.5)