FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600885
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2020253.0605744532740684Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG575560.8719436863390289No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC80800.12240782864721927Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA31550.045.4842747
GTCCCCG28700.044.63503645
TCCCCGT32450.040.9866446
AGAGCAC38250.040.90401515
GGTCCCC33750.040.02890844
GTGGTCC33500.040.01208542
TGGTCCC34550.039.5051543
GTGACTG30350.039.3644071
GACCGAC14100.038.72329310
GAGCACA36300.038.1828716
TGACTGG32200.038.068762
GACTAGT27950.037.82000412
GACTGGA35100.037.2091373
AGATAGA98500.037.026687
AAGACCG35500.036.676238
CCGACGT15750.036.66906412
CCCGTAA18900.036.4822148
CGATCGG8650.036.45311
GGATCGG20050.035.8218081
AGACTAG27650.034.81002811
Produced by FastQC (version 0.11.5)