FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10283824
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2174852.114826158051713Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG853810.8302456362535959No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC66900.040.5482415
GAGCACA62450.039.45583316
CCCCGTA35500.038.9446947
GTCCCCG32150.038.7568545
GTGACTG44050.035.872211
GGTCCCC35700.035.5879644
TCCCCGT37250.034.29620446
GACTGGA53500.033.9017683
TGGTCCC38000.033.89200243
TGACTGG48700.033.803292
GTGGTCC38600.033.0025242
AGATAGA138250.032.8617867
AAAGGGG486150.030.57393367
GATCGAG28350.030.122938
AAGGGGG511850.029.509668
GATAGAT138450.029.2735988
GACCGAC15700.029.2030310
ACTGGAG63300.028.811674
CGTGGTC40400.027.72350347
CCGTGGT39550.027.69984446
Produced by FastQC (version 0.11.5)