FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10888180
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3127242.872142084352022Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG798130.7330242519870172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA53450.048.71830747
GTCCCCG45700.047.71301745
GGTCCCC50200.045.6661744
TCCCCGT50100.044.7100646
GTGGTCC52000.043.67817342
TGGTCCC53950.043.65627743
GACCGAC21500.041.1848110
AGAGCAC67400.041.18207615
GAGCACA63300.040.254716
GTGACTG52150.039.9759451
CCGACGT24750.038.89236512
TGACTGG56050.038.8691522
CCCGTAA33650.038.6923348
ACCGACG25950.037.3595311
GACTAGT53700.037.08897812
GTACCCC26300.036.86218344
GACTGGA63400.036.5075533
AAGACCG55550.036.353718
CCCGTAT41050.035.2130748
AGATAGA171150.035.010147
Produced by FastQC (version 0.11.5)