FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n02_ncm4-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n02_ncm4-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11668245
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3706403.1764845527326515Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG958100.8211174859629704No Hit
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC140310.12024944625348544Illumina Single End PCR Primer 1 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC75650.043.44591515
CCCCGTA56550.043.13937847
GTCCCCG51500.042.8160945
GAGCACA70050.042.62066316
GGTCCCC57700.041.73203744
GTGACTG53150.040.6735341
TGGTCCC60200.040.5782743
AGATAGA189800.039.813997
GTGGTCC59750.039.77082442
GACCGAC20300.038.96502310
TCCCCGT54850.038.79722246
TGACTGG59400.037.799022
GATCGAG38800.037.3452038
GACTGGA66600.037.276863
GATAGAT183150.036.8439648
AAAGGGG828950.035.88539567
GACCCCG13800.035.50770645
AAGGGGG849350.035.25789668
ACCGACG24550.034.78585411
CAGATAG73450.034.597346