Basic Statistics
Measure | Value |
---|---|
Filename | HTFH5AFXY_n02_ncm4-11.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11668245 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 41 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC | 370640 | 3.1764845527326515 | Illumina Single End PCR Primer 1 (100% over 50bp) |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 95810 | 0.8211174859629704 | No Hit |
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC | 14031 | 0.12024944625348544 | Illumina Single End PCR Primer 1 (100% over 49bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AGAGCAC | 7565 | 0.0 | 43.445915 | 15 |
CCCCGTA | 5655 | 0.0 | 43.139378 | 47 |
GTCCCCG | 5150 | 0.0 | 42.81609 | 45 |
GAGCACA | 7005 | 0.0 | 42.620663 | 16 |
GGTCCCC | 5770 | 0.0 | 41.732037 | 44 |
GTGACTG | 5315 | 0.0 | 40.673534 | 1 |
TGGTCCC | 6020 | 0.0 | 40.57827 | 43 |
AGATAGA | 18980 | 0.0 | 39.81399 | 7 |
GTGGTCC | 5975 | 0.0 | 39.770824 | 42 |
GACCGAC | 2030 | 0.0 | 38.965023 | 10 |
TCCCCGT | 5485 | 0.0 | 38.797222 | 46 |
TGACTGG | 5940 | 0.0 | 37.79902 | 2 |
GATCGAG | 3880 | 0.0 | 37.345203 | 8 |
GACTGGA | 6660 | 0.0 | 37.27686 | 3 |
GATAGAT | 18315 | 0.0 | 36.843964 | 8 |
AAAGGGG | 82895 | 0.0 | 35.885395 | 67 |
GACCCCG | 1380 | 0.0 | 35.507706 | 45 |
AAGGGGG | 84935 | 0.0 | 35.257896 | 68 |
ACCGACG | 2455 | 0.0 | 34.785854 | 11 |
CAGATAG | 7345 | 0.0 | 34.59734 | 6 |