HTFH5AFXY_n02_ncm4-10.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HTFH5AFXY_n02_ncm4-10.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7053430
Sequences flagged as poor quality	0
Sequence length	76
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	269972	3.8275278835970585	Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	75091	1.0646026117789502	No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTCTCGGTGGTCGCC	10368	0.14699231437754398	Illumina Single End PCR Primer 1 (98% over 50bp)
AGAACGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	9052	0.12833472509119676	Illumina Single End PCR Primer 1 (98% over 50bp)
AGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCC	8961	0.1270445726405451	Illumina Single End PCR Primer 1 (98% over 50bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC	7143	0.10126987862642714	Illumina Single End PCR Primer 1 (100% over 49bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CCCCGTA	3690	51.31591	47
GTCCCCG	3200	51.07966	45
GTGACTG	4675	50.432316	1
TCCCCGT	3395	49.589115	46
TGGTCCC	3465	49.39157	43
GGTCCCC	3465	48.585594	44
TGACTGG	5020	48.210876	2
CCCGTAA	2505	46.807796	48
GTGGTCC	3725	46.03774	42
AGATAGA	18980	45.956646	7
GACTGGA	6140	43.856415	3
CGCCGTA	82465	43.6659	47
CCGACGT	1975	43.59903	12
TCGCCGT	83075	43.32842	46
GATAGAT	17625	43.173847	8
GACCGAC	1785	42.942165	10
CAGATAG	6780	42.799004	6
CCGTATC	80490	42.197815	49
GAGCACA	3650	41.903885	16
AAGACCG	5245	40.907726	8