FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30326402
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8383162.7643107810811185No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2974720.9809010643596955No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA2534600.8357733963956554TruSeq Adapter, Index 14 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1390750.45859380219255813TruSeq Adapter, Index 9 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG1282130.4227768266080493TruSeq Adapter, Index 11 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG1040090.34296518261546494TruSeq Adapter, Index 3 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG1011130.33341574776988053TruSeq Adapter, Index 2 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG769590.2537689766164809TruSeq Adapter, Index 10 (100% over 49bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG755680.2491822142303594No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG742320.24477681196734122TruSeq Adapter, Index 12 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG684810.22581313800430397TruSeq Adapter, Index 8 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATG637090.21007767423250537TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG575180.1896631192846418TruSeq Adapter, Index 4 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATG547580.1805621385616401TruSeq Adapter, Index 22 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG516760.17039937675428823TruSeq Adapter, Index 13 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG475950.15694245562002376TruSeq Adapter, Index 5 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATG433040.1427930685611831TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATG311440.1026959940714365TruSeq Adapter, Index 20 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG3352550.058.7454570
AGGGGGG406500.055.2088931
GGATCGG199450.051.8170431
ACACTCT416650.050.146591
CACTCTT496700.047.856122
CCTACAC541850.046.17080711
GGGATCT99450.046.1461538
TCCGGCT148300.046.0418756
TCCCTAC541100.046.0346229
CCCTACA544350.045.8625210
AAAAAAG4393000.045.72189369
CGACGCT530800.045.55335217
TCACGGG75750.045.26505731
TACACGA551400.044.55853313
CTACACG567250.044.1899712
CTTTCCC563800.044.0207526
TTCCCTA564050.044.019188
ACTCTTT543550.043.8529623
GACGCTC547000.043.12663318
GGTCGGA110300.042.4987342
Produced by FastQC (version 0.11.5)