FastQCFastQC Report
Wed 2 Oct 2019
HTFH5AFXY_n01_ncm4-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTFH5AFXY_n01_ncm4-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876014
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG3879326.601958402413609TruSeq Adapter, Index 10 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1012811.7236344229268346No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG638701.086961331269803TruSeq Adapter, Index 10 (97% over 49bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG315490.536911586664021No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATG315430.5368094766282041TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGGATG259520.44165994158625216TruSeq Adapter, Index 10 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATT251610.42819843519773776TruSeq Adapter, Index 10 (97% over 48bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGTATG184600.3141585435296784TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGGATG172960.29434919658121983TruSeq Adapter, Index 10 (97% over 45bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTT171070.29113273045299076TruSeq Adapter, Index 10 (100% over 39bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169240.2880183743605784No Hit
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG168750.28718447573474126TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTTTG147870.251650183270496TruSeq Adapter, Index 10 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC117560.20006759684371073TruSeq Adapter, Index 10 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATG101800.17324669410249874TruSeq Adapter, Index 10 (97% over 42bp)
TGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG96250.16380151578944502TruSeq Adapter, Index 10 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGATG68800.11708617440326044TruSeq Adapter, Index 10 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTTTG64710.11012567362841547TruSeq Adapter, Index 10 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCGTATCTCGTATG59060.10051031192233374TruSeq Adapter, Index 10 (97% over 49bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTAT58850.10015292679697496TruSeq Adapter, Index 10 (100% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT166950.062.738591
AAAAAGG822900.062.3099870
GGATCGG59450.061.5941431
CACTCTT199650.056.3430142
TCCGGCT106850.054.7027556
AGGGGGG45500.054.319141
ACTCTTT207100.054.3152583
TGATCGG36000.052.1220971
CCCTACA221700.051.73114410
TCCCTAC222100.051.6694959
CCTACAC222350.051.5484411
CTTTCCC222050.051.524836
GCTTGTA100950.051.5204560
TCTTTCC221600.050.8867235
CTACACG225250.050.72939312
TACACGA223900.050.65969513
ACGACGC223750.050.55288316
CGACGCT221950.050.5371217
CTCTTTC223250.050.526314
CACGACG225600.050.40205815
Produced by FastQC (version 0.11.5)